PMID- 16706844
OWN - NLM
STAT- MEDLINE
DCOM- 20061006
LR  - 20161128
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 23
IP  - 9
DP  - 2006 May
TI  - Spike-timing-dependent plasticity at resting and conditioned lateral perforant 
      path synapses on granule cells in the dentate gyrus: different roles of 
      N-methyl-D-aspartate and group I metabotropic glutamate receptors.
PG  - 2362-74
AB  - We examined the mechanisms underlying spike-timing-dependent plasticity induction 
      at resting and conditioned lateral perforant pathway (LPP) synapses in the rat 
      dentate gyrus. Two stimulating electrodes were placed in the outer third of the 
      molecular layer and in the granule cell layer in hippocampal slices to evoke 
      field excitatory postsynaptic potentials (fEPSPs) and antidromic field somatic 
      spikes (afSSs), respectively. Long-term potentiation (LTP) of LPP synapses was 
      induced by paired stimulation with fEPSP preceding afSS. Reversal of the temporal 
      order of fEPSP and afSS stimulation resulted in long-term depression (LTD). 
      Induction of LTP or LTD was blocked by D,L-2-amino-5-phosphonopentanoic acid 
      (AP5), showing that both effects were N-methyl-D-aspartate receptor 
      (NMDAR)-dependent. Induction of LTP was also blocked by inhibitors of 
      calcium-calmodulin kinase II, protein kinase C or 
      mitogen-activated/extracellular-signal regulated kinase, suggesting that these 
      are downstream effectors of NMDAR activation, whereas induction of LTD was 
      blocked by inhibitors of protein kinase C and protein phosphatase 2B. At LPP 
      synapses previously potentiated by high-frequency stimulation or depressed by 
      low-frequency stimulation, paired fEPSP-afSS stimulation resulted in 
      'de-depression' at depressed LPP synapses but had no effect on potentiated 
      synapses, whereas reversal of the temporal order of fEPSP-afSS stimulation 
      resulted in 'de-potentiation' at potentiated synapses but had no effect on 
      depressed synapses. Induction of de-depression and de-potentiation was unaffected 
      by ap5 but was blocked by 2-methyl-6-(phenylethynyl) pyridine hydrochloride, a 
      group I metabotropic glutamate receptor blocker, showing that both were 
      NMDAR-independent but group I metabotropic glutamate receptor-dependent. In 
      conclusion, our results show that spike-timing-dependent plasticity can occur at 
      both resting and conditioned LPP synapses, its induction in the former case being 
      NMDAR-dependent and, in the latter, group I metabotropic glutamate 
      receptor-dependent.
FAU - Lin, Yi-Wen
AU  - Lin YW
AD  - Department of Life Science, College of Life Science, National Taiwan University, 
      1 Sec.4 Roosevelt Road, Taipei 106, Taiwan.
FAU - Yang, Hsiu-Wen
AU  - Yang HW
FAU - Wang, Hui-Ju
AU  - Wang HJ
FAU - Gong, Chi-Li
AU  - Gong CL
FAU - Chiu, Tsai-Hsien
AU  - Chiu TH
FAU - Min, Ming-Yuan
AU  - Min MY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 62T278S1MX (FG 9041)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Dentate Gyrus/*cytology
MH  - Electric Stimulation/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology/radiation effects
MH  - In Vitro Techniques
MH  - Male
MH  - Membrane Potentials/drug effects/physiology/radiation effects
MH  - Neuronal Plasticity/drug effects/*physiology/radiation effects
MH  - Neurons/drug effects/*physiology
MH  - Perforant Pathway/*cytology/drug effects/radiation effects
MH  - Quinoxalines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Metabotropic Glutamate/antagonists & inhibitors/*physiology
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/*physiology
MH  - Synapses/drug effects/*physiology/radiation effects
MH  - Tetrodotoxin/pharmacology
MH  - Time Factors
EDAT- 2006/05/19 09:00
MHDA- 2006/10/07 09:00
CRDT- 2006/05/19 09:00
PHST- 2006/05/19 09:00 [pubmed]
PHST- 2006/10/07 09:00 [medline]
PHST- 2006/05/19 09:00 [entrez]
AID - EJN4730 [pii]
AID - 10.1111/j.1460-9568.2006.04730.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 May;23(9):2362-74. doi: 10.1111/j.1460-9568.2006.04730.x.