PMID- 16707102
OWN - NLM
STAT- MEDLINE
DCOM- 20060801
LR  - 20220318
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 345
IP  - 3
DP  - 2006 Jul 7
TI  - Establishment of primary cultures for mouse ameloblasts as a model of their 
      lifetime.
PG  - 1247-53
AB  - To understand how the properties of ameloblasts are spatiotemporally regulated 
      during amelogenesis, two primary cultures of ameloblasts in different stages of 
      differentiation were established from mouse enamel epithelium. Mouse primary 
      ameloblasts (MPAs) prepared from immature enamel epithelium (MPA-I) could 
      proliferate, whereas those from mature enamel epithelium (MPA-M) could not. MPA-M 
      but not MPA-I caused apoptosis during culture. The mRNA expression of amelogenin, 
      a marker of immature ameloblasts, was down-regulated, and that of enamel matrix 
      serine proteiase-1, a marker of mature ameloblasts, was induced in MPA-I during 
      culture. Using green fluorescence protein as a reporter, a visualized reporter 
      system was established to analyze the promoter activity of the amelogenin gene. 
      The region between -1102bp and -261bp was required for the reporter expression in 
      MPA-I. These results suggest that MPAs are valuable in vitro models for 
      investigation of ameloblast biology, and that the visualized system is useful for 
      promoter analysis in MPAs.
FAU - Suzawa, Tetsuo
AU  - Suzawa T
AD  - Department of Biochemistry, School of Dentistry, Showa University, Tokyo 
      142-8555, Japan. suzawa@dent.showa-u.ac.jp
FAU - Itoh, Nao
AU  - Itoh N
FAU - Takahashi, Naoyuki
AU  - Takahashi N
FAU - Katagiri, Takenobu
AU  - Katagiri T
FAU - Morimura, Naoko
AU  - Morimura N
FAU - Kobayashi, Yasuna
AU  - Kobayashi Y
FAU - Yamamoto, Toshinori
AU  - Yamamoto T
FAU - Kamijo, Ryutaro
AU  - Kamijo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060502
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Ameloblasts/*cytology
MH  - Animals
MH  - Apoptosis
MH  - Bromodeoxyuridine/pharmacology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dental Enamel/*cytology
MH  - Gene Deletion
MH  - Genes, Reporter
MH  - In Situ Nick-End Labeling
MH  - Mice
MH  - Promoter Regions, Genetic
EDAT- 2006/05/19 09:00
MHDA- 2006/08/02 09:00
CRDT- 2006/05/19 09:00
PHST- 2006/03/29 00:00 [received]
PHST- 2006/04/17 00:00 [accepted]
PHST- 2006/05/19 09:00 [pubmed]
PHST- 2006/08/02 09:00 [medline]
PHST- 2006/05/19 09:00 [entrez]
AID - S0006-291X(06)00932-6 [pii]
AID - 10.1016/j.bbrc.2006.04.122 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Jul 7;345(3):1247-53. doi: 
      10.1016/j.bbrc.2006.04.122. Epub 2006 May 2.