PMID- 16707256
OWN - NLM
STAT- MEDLINE
DCOM- 20070531
LR  - 20220321
IS  - 0944-7113 (Print)
IS  - 0944-7113 (Linking)
VI  - 14
IP  - 4
DP  - 2007 Apr
TI  - Protective effect of safranal on pentylenetetrazol-induced seizures in the rat: 
      involvement of GABAergic and opioids systems.
PG  - 256-62
AB  - The aim of the present study was to evaluate the effects of safranal, an active 
      constituent of Crocus sativus L. stigmas, on seizures induced by 
      pentylenetetrazol. Intracerebroventricular (i.c.v.) microinjection of safranal 
      (4.84, 9.68 and 24.2 micromol) had no effects on tonic and clonic phases as well 
      as mortality upon seizures induced by PTZ (90mg/kg body wt., i.p.). Peripheral 
      administration of safranal (72.75, 145.5 and 291 mg/kg body wt., i.p.), however, 
      induced a dose-dependent decrease in the incidence of both minimal clonic 
      seizures (MCS) (145.5 mg/kg body wt., p<0.01) and generalized tonic-clonic 
      seizures (GTCS) (145.5 mg/kg body wt., p<0.001) following PTZ administration. 
      Safranal also increased MCS and GTCS latency, significantly. Percent of 
      protection against GTCS was 30%, 100% and 100% and mortality protection percent 
      was 40%, 100% and 100% for the mentioned doses, respectively. Pretreatment with 
      flumazenil (5 nmol, i.c.v.) and naloxone (5.5 nmol, i.c.v. and 2 mg/kg body wt., 
      i.p.), 15 min prior to safranal administration (145.5 mg/kg body wt., i.p.), 
      abolished the protective effect of safranal on MCS. Flumazenil also decreased the 
      effect of safranal on incidence as well as latency of GTCS, significantly. These 
      effects were not, however, significant for naloxone (5.5 nmol, i.c.v. and 2mg/kg 
      body wt., i.p.). Results of this study demonstrated that safranal could exert 
      anticonvulsant activity in the PTZ model and this effect may be mediated, at 
      least partly, through GABA(A)-benzodiazepine receptor complex.
FAU - Hosseinzadeh, H
AU  - Hosseinzadeh H
AD  - Faculty of Pharmacy, Pharmaceutical Research Center, Mashhad University of 
      Medical Sciences, Mashhad, I.R. Iran. hosseinzadehh@mums.ac.ir
FAU - Sadeghnia, H R
AU  - Sadeghnia HR
LA  - eng
PT  - Journal Article
DEP - 20060516
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Anticonvulsants)
RN  - 0 (Cyclohexenes)
RN  - 0 (Plant Extracts)
RN  - 0 (Terpenes)
RN  - 4393FR07EA (safranal)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Anticonvulsants/administration & dosage/*pharmacology/therapeutic use
MH  - Cerebral Ventricles
MH  - *Crocus
MH  - Cyclohexenes/administration & dosage/*pharmacology/therapeutic use
MH  - Dose-Response Relationship, Drug
MH  - Epilepsy/drug therapy
MH  - Injections
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Pentylenetetrazole
MH  - *Phytotherapy
MH  - Plant Extracts/administration & dosage/pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Terpenes/administration & dosage/*pharmacology/therapeutic use
EDAT- 2006/05/19 09:00
MHDA- 2007/06/01 09:00
CRDT- 2006/05/19 09:00
PHST- 2006/05/19 09:00 [pubmed]
PHST- 2007/06/01 09:00 [medline]
PHST- 2006/05/19 09:00 [entrez]
AID - S0944-7113(06)00065-1 [pii]
AID - 10.1016/j.phymed.2006.03.007 [doi]
PST - ppublish
SO  - Phytomedicine. 2007 Apr;14(4):256-62. doi: 10.1016/j.phymed.2006.03.007. Epub 
      2006 May 16.