PMID- 16709251
OWN - NLM
STAT- MEDLINE
DCOM- 20060809
LR  - 20181113
IS  - 1476-511X (Electronic)
IS  - 1476-511X (Linking)
VI  - 5
DP  - 2006 May 18
TI  - Caloric restriction in C57BL/6J mice mimics therapeutic fasting in humans.
PG  - 13
AB  - BACKGROUND: Caloric restriction (CR) has long been recognized as a dietary 
      therapy that improves health and increases longevity. Little is known about the 
      persistent effects of CR on plasma biomarkers (glucose, ketone bodies, and 
      lipids) following re-feeding in mice. It is also unclear how these biomarker 
      changes in calorically restricted mice relate to those observed previously in 
      calorically restricted humans. RESULTS: Three groups of individually housed adult 
      female C57BL/6J (B6) mice (n = 4/group) were fed a standard rodent chow diet 
      either: (1) unrestricted (UR); (2) restricted for three weeks to reduce body 
      weight by approximately 15-20% (R); or (3) restricted for three weeks and then 
      re-fed unrestricted (ad libitum) for an additional three weeks (R-RF). Body 
      weight and food intake were measured throughout the study, while plasma lipids 
      and levels of glucose and ketone bodies (beta-hydroxybutyrate) were measured at 
      the termination of the study. Plasma glucose, phosphatidylcholine, cholesterol, 
      and triglycerides were significantly lower in the R mice than in the UR mice. In 
      contrast, plasma fatty acids and beta-hydroxybutyrate were significantly higher 
      in the R mice than in the UR mice. CR had no effect on plasma 
      phosphatidylinositol levels. While body weight and plasma lipids of the R-RF mice 
      returned to unrestricted levels upon re-feeding, food intake and glucose levels 
      remained significantly lower than those prior to the initiation of CR. 
      CONCLUSION: CR establishes a new homeostatic state in B6 mice that persists for 
      at least three weeks following ad libitum re-feeding. Moreover, the plasma 
      biomarker changes observed in B6 mice during CR mimic those reported in humans on 
      very low calorie diets or during therapeutic fasting.
FAU - Mahoney, Lisa B
AU  - Mahoney LB
AD  - Biology Department, Boston College, Chestnut Hill, MA, USA. mahoneli@bc.edu
FAU - Denny, Christine A
AU  - Denny CA
FAU - Seyfried, Thomas N
AU  - Seyfried TN
LA  - eng
GR  - R01 CA102135/CA/NCI NIH HHS/United States
GR  - R01 HD039722/HD/NICHD NIH HHS/United States
GR  - CA102135/CA/NCI NIH HHS/United States
GR  - HD39722/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060518
PL  - England
TA  - Lipids Health Dis
JT  - Lipids in health and disease
JID - 101147696
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Lipids)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/blood
MH  - Animals
MH  - Biomarkers/blood
MH  - Blood Glucose/metabolism
MH  - Body Weight/physiology
MH  - *Caloric Restriction
MH  - Cholesterol/blood
MH  - Eating/physiology
MH  - Fasting/*blood
MH  - Female
MH  - Homeostasis/*physiology
MH  - Humans
MH  - Lipids/blood
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphatidylcholines/blood
MH  - Triglycerides/blood
PMC - PMC1513228
EDAT- 2006/05/20 09:00
MHDA- 2006/08/10 09:00
PMCR- 2006/05/18
CRDT- 2006/05/20 09:00
PHST- 2006/03/27 00:00 [received]
PHST- 2006/05/18 00:00 [accepted]
PHST- 2006/05/20 09:00 [pubmed]
PHST- 2006/08/10 09:00 [medline]
PHST- 2006/05/20 09:00 [entrez]
PHST- 2006/05/18 00:00 [pmc-release]
AID - 1476-511X-5-13 [pii]
AID - 10.1186/1476-511X-5-13 [doi]
PST - epublish
SO  - Lipids Health Dis. 2006 May 18;5:13. doi: 10.1186/1476-511X-5-13.