PMID- 16709674
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 103
IP  - 22
DP  - 2006 May 30
TI  - Postsynaptic inositol 1,4,5-trisphosphate signaling maintains presynaptic 
      function of parallel fiber-Purkinje cell synapses via BDNF.
PG  - 8528-33
AB  - The maintenance of synaptic functions is essential for neuronal information 
      processing, but cellular mechanisms that maintain synapses in the adult brain are 
      not well understood. Here, we report an activity-dependent maintenance mechanism 
      of parallel fiber (PF)-Purkinje cell (PC) synapses in the cerebellum. When 
      postsynaptic metabotropic glutamate receptor (mGluR) or inositol 
      1,4,5-trisphosphate (IP(3)) signaling was chronically inhibited in vivo, PF-PC 
      synaptic strength decreased because of a decreased transmitter release 
      probability. The same effects were observed when PF activity was inhibited in 
      vivo by the suppression of NMDA receptor-mediated inputs to granule cells. PF-PC 
      synaptic strength similarly decreased after the in vivo application of an 
      antibody against brain-derived neurotrophic factor (BDNF). Furthermore, the 
      weakening of synaptic connection caused by the blockade of mGluR-IP(3) signaling 
      was reversed by the in vivo application of BDNF. These results indicate that a 
      signaling cascade comprising PF activity, postsynaptic mGluR-IP(3) signaling and 
      subsequent BDNF signaling maintains presynaptic functions in the mature 
      cerebellum.
FAU - Furutani, Kazuharu
AU  - Furutani K
AD  - Department of Pharmacology, Graduate School of Medicine, University of Tokyo, 
      Tokyo 113-0033, Japan.
FAU - Okubo, Yohei
AU  - Okubo Y
FAU - Kakizawa, Sho
AU  - Kakizawa S
FAU - Iino, Masamitsu
AU  - Iino M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060518
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 3.1.3.- (inositol triphosphate phosphomonoesterase)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Electrophysiology
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphoric Monoester Hydrolases/genetics/metabolism
MH  - Purkinje Cells/*metabolism
MH  - Receptors, Metabotropic Glutamate/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - *Signal Transduction
MH  - Synapses/*metabolism
PMC - PMC1482525
COIS- Conflict of interest statement: No conflicts declared.
EDAT- 2006/05/20 09:00
MHDA- 2006/07/27 09:00
PMCR- 2006/11/18
CRDT- 2006/05/20 09:00
PHST- 2006/05/20 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/05/20 09:00 [entrez]
PHST- 2006/11/18 00:00 [pmc-release]
AID - 0600497103 [pii]
AID - 2355 [pii]
AID - 10.1073/pnas.0600497103 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2006 May 30;103(22):8528-33. doi: 
      10.1073/pnas.0600497103. Epub 2006 May 18.