PMID- 16709677
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20240109
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 103
IP  - 22
DP  - 2006 May 30
TI  - A unique strategy for mRNA cap methylation used by vesicular stomatitis virus.
PG  - 8493-8
AB  - Nonsegmented negative-sense (nsNS) RNA viruses typically replicate within the 
      host cell cytoplasm and do not have access to the host mRNA capping machinery. 
      These viruses have evolved a unique mechanism for mRNA cap formation in that the 
      guanylyltransferase transfers GDP rather than GMP onto the 5' end of the RNA. 
      Working with vesicular stomatitis virus (VSV), a prototype nsNS RNA virus, we now 
      provide genetic and biochemical evidence that its mRNA cap methylase activities 
      are also unique. Using recombinant VSV, we determined the function in mRNA cap 
      methylation of a predicted binding site in the polymerase for the methyl donor, 
      S-adenosyl-l-methionine. We found that amino acid substitutions to this site 
      disrupted methylation at the guanine-N-7 (G-N-7) position or at both the G-N-7 
      and ribose-2'-O (2'-O) positions of the mRNA cap. These studies provide genetic 
      evidence that the two methylase activities share an 
      S-adenosyl-l-methionine-binding site and show that, in contrast to other cap 
      methylation reactions, methylation of the G-N-7 position is not required for 2'-O 
      methylation. These findings suggest that VSV evolved an unusual strategy of mRNA 
      cap methylation that may be shared by other nsNS RNA viruses.
FAU - Li, Jianrong
AU  - Li J
AD  - Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 
      Longwood Avenue, Boston, MA 02115, USA.
FAU - Wang, Jennifer T
AU  - Wang JT
FAU - Whelan, Sean P J
AU  - Whelan SP
LA  - eng
GR  - R01 AI059371/AI/NIAID NIH HHS/United States
GR  - R01 AI059371-02/AI/NIAID NIH HHS/United States
GR  - R37 AI059371/AI/NIAID NIH HHS/United States
GR  - AI 059371/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060518
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (RNA Caps)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Proteins)
RN  - 5Z93L87A1R (Guanine)
RN  - EC 2.1.1.- (tRNA Methyltransferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - Cricetinae
MH  - Gene Expression Regulation, Viral
MH  - Guanine/metabolism
MH  - Methylation
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Phenotype
MH  - RNA Caps/genetics/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Vesicular stomatitis Indiana virus/chemistry/*genetics/*metabolism
MH  - Viral Proteins/chemistry/genetics/metabolism
MH  - tRNA Methyltransferases/chemistry/genetics/metabolism
PMC - PMC1482520
COIS- Conflict of interest statement: No conflicts declared.
EDAT- 2006/05/20 09:00
MHDA- 2006/07/27 09:00
PMCR- 2006/11/18
CRDT- 2006/05/20 09:00
PHST- 2006/05/20 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/05/20 09:00 [entrez]
PHST- 2006/11/18 00:00 [pmc-release]
AID - 0509821103 [pii]
AID - 2353 [pii]
AID - 10.1073/pnas.0509821103 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2006 May 30;103(22):8493-8. doi: 
      10.1073/pnas.0509821103. Epub 2006 May 18.