PMID- 16711000
OWN - NLM
STAT- MEDLINE
DCOM- 20061013
LR  - 20191110
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 26
IP  - 1
DP  - 2006
TI  - Correlation between endotoxin tolerance in human monocyte leukemia cell line 
      THP-1 with glucocorticoid receptor-alpha.
PG  - 28-30
AB  - Human monocyte leukemia cell line THP-1 was stimulated with lipopolysaccharide 
      (LPS) to simulate the sepsis model and the expression of human glucocorticoid 
      receptor-alpha (GR-alpha) mRNA in montocytes with endotoxin tolerance was 
      investigated. THP-1 cells were cultured in serum-free medium, randomly divided 
      into groups A, B, C, D and E, and stimulated with 0, 10, 10, 100, 0 ng/mL LPS for 
      24 h followed with 100, 100, 10, 100, 0 ng/mL LPS for another 24 h respectively. 
      The expression of GR-alpha mRNA was detected by semi-quantitative reverse 
      transcriptional polymerase chain reaction. Tumor necrosis factor-alpha 
      (TNF-alpha) was determined by enzyme linked immunosorbent assay (ELISA). The 
      results showed that the A values of GR-alpha/beta-actin in groups A, B, C, D and 
      E was 0.607 +/- 0.006, 0.368 +/- 0.005, 0.484 +/- 0.008, 0.509 +/- 0.004 and 
      0.564 +/- 0.014 respectively with the difference being significant among the 
      groups (P < 0.05). The GR-alpha mRNA expression was negatively correlated with 
      the TNF-alpha expression (P < 0.01). It was concluded that the down-regulation of 
      the expression of GR-alpha mRNA in endotoxin tolerance THP-1 cells might play an 
      important role in the development of endotoxin tolerance in THP-1 cells.
FAU - Yang, Zhaohui
AU  - Yang Z
AD  - Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, China.
FAU - Bai, Xiangjun
AU  - Bai X
FAU - Wang, Haiping
AU  - Wang H
FAU - Li, Zhanfei
AU  - Li Z
FAU - Li, Siqi
AU  - Li S
FAU - Li, Bo
AU  - Li B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Endotoxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (glucocorticoid receptor alpha)
SB  - IM
MH  - Drug Tolerance
MH  - Endotoxins/*pharmacology
MH  - Humans
MH  - Leukemia, Monocytic, Acute/*metabolism/pathology
MH  - Monocytes/*metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptors, Glucocorticoid/*biosynthesis/genetics
MH  - Tumor Cells, Cultured
EDAT- 2006/05/23 09:00
MHDA- 2006/10/14 09:00
CRDT- 2006/05/23 09:00
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/10/14 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - 10.1007/BF02828030 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2006;26(1):28-30. doi: 10.1007/BF02828030.