PMID- 16712645
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20061115
IS  - 1744-3121 (Print)
IS  - 1744-3121 (Linking)
VI  - 33
IP  - 3
DP  - 2006 Jun
TI  - ELISA methods detect HLA antibodies with variable sensitivity.
PG  - 163-6
AB  - The accuracy of human leukocyte antigen (HLA) antibody screening results is 
      dependent on the technique employed. In this study, a total of 612 serum samples 
      from patients awaiting kidney transplantation were tested by two different 
      enzyme-linked immunosorbent assay (ELISA) methods: LAT-M(R) (One Lambda/BMT) and 
      AbScreen(R) (Biotest). The results of these assays were identical for HLA class I 
      and II antibodies in 524 cases (85.6%), and discrepant in the remaining 88 cases 
      (42 class I, 24 class II, 22 class I and II). More specifically, class I results 
      were in agreement in 26 positive and 522 negative cases and in disagreement in 64 
      cases. Class II results were the same in 50 positive and 516 negative cases and 
      different in 46 cases. Retesting the samples using LAT 1288 (One Lambda/BMT) and 
      considering previous HLA antibody test results and the history of immunising 
      events, the sensitivity and specificity, respectively, of the two methods were 
      determined as follows: class I: 89.6% and 97.5% (LAT-M) vs. 70.6% and 96.8% 
      (AbScreen), class II: 96.5% and 98.4% (LAT-M) vs. 88.3% and 95.5% (AbScreen). 
      These results indicate that a single ELISA does not invariably prove or exclude 
      the presence of HLA antibodies, and that additional testing is required in some 
      cases.
FAU - Monien, S
AU  - Monien S
AD  - Tissue Typing Laboratory, Institute of Transfusion Medicine, Charite, University 
      Hospital Berlin, Germany. silke.monien@charite.de
FAU - Salama, A
AU  - Salama A
FAU - Schonemann, C
AU  - Schonemann C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Immunogenet
JT  - International journal of immunogenetics
JID - 101232337
RN  - 0 (Autoantibodies)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - *Antibody Specificity
MH  - Autoantibodies/*blood
MH  - Enzyme-Linked Immunosorbent Assay/methods/standards
MH  - Female
MH  - Histocompatibility
MH  - *Histocompatibility Antigens Class I
MH  - *Histocompatibility Antigens Class II
MH  - Humans
MH  - Kidney Transplantation
MH  - Male
MH  - Reagent Kits, Diagnostic/standards
MH  - Sensitivity and Specificity
EDAT- 2006/05/23 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/05/23 09:00
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - EJI586 [pii]
AID - 10.1111/j.1744-313X.2006.00586.x [doi]
PST - ppublish
SO  - Int J Immunogenet. 2006 Jun;33(3):163-6. doi: 10.1111/j.1744-313X.2006.00586.x.