PMID- 16712839
OWN - NLM
STAT- MEDLINE
DCOM- 20061109
LR  - 20071115
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 83
IP  - 4
DP  - 2006 Oct
TI  - Thioredoxin induced antioxidant gene expressions in human lens epithelial cells.
PG  - 783-92
AB  - Thioredoxin (Trx) is one of the major redox-regulating proteins. It catalyzes 
      dithiol/disulfide exchange reactions and displays many unique intracellular and 
      extracellular activities thereby controlling multiple mammalian cell functions. 
      In the present study we examine the effect of exogenous Trx on the expression of 
      several antioxidant genes in human lens epithelial (HLE B3) cells. mRNA levels 
      for gene expression were monitored by RT-PCR and real-time PCR while protein 
      levels were measured by western blot analysis. We have found that recombinant 
      human Trx (hTrx)-treated HLE B3 cells have a simultaneous increase in mRNA 
      expressions of mitochondrial manganese superoxide dismutase (MnSOD), 
      thioltranferase 1 (TTase 1) or glutaredoxin 1 (Grx1), mitochondrial 
      thioltransferase (TTase 2) or glutaredoxin 2 (Grx2), and thioredoxin peroxidase 
      IV (Prx IV). The increased MnSOD and TTase 1 mRNA expressions were accompanied 
      with their respective increases in protein levels. Other antioxidant genes, 
      including Cu/ZnSOD, catalase, glutathione peroxidase 1 (GPx1), thioredoxin 
      reductase 1 (TrxR1), thioredoxin peroxidase III (Prx III), and gamma-glutamyl 
      cysteine synthetase were not affected. The ability of Trx to induce selectively 
      these antioxidant genes in the absence of oxidative stress suggest a 
      cytokine/growth factor-like new physiological role of hTrx in HLE B3 cells. Our 
      data also provide evidence of a strong antioxidant defense system in HLE B3 cells 
      that can be activated by extracellular hTrx, as well as of a possible link 
      between the thioredoxin (Trx) and glutathione (GSH) redox regulating systems in 
      these cells.
FAU - Yegorova, Svitlana
AU  - Yegorova S
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, 
      134 VBS, Fair St./East Campus Loop, 68583-0905, USA.
FAU - Yegorov, Oleg
AU  - Yegorov O
FAU - Lou, Marjorie F
AU  - Lou MF
LA  - eng
GR  - R01EY10595/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060519
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Antioxidants)
RN  - 0 (GLRX2 protein, human)
RN  - 0 (Glutaredoxins)
RN  - 0 (Recombinant Proteins)
RN  - 52500-60-4 (Thioredoxins)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.4.2 (Protein Disulfide Reductase (Glutathione))
SB  - IM
MH  - Antioxidants/*metabolism
MH  - Cells, Cultured
MH  - Epithelial Cells/drug effects/enzymology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Glutaredoxins
MH  - Humans
MH  - Lens, Crystalline/*drug effects/enzymology
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Oxidoreductases/biosynthesis/genetics
MH  - Peroxidases/biosynthesis/genetics
MH  - Peroxiredoxins
MH  - Protein Disulfide Reductase (Glutathione)/biosynthesis/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Superoxide Dismutase/biosynthesis/genetics
MH  - Thioredoxins/*pharmacology
EDAT- 2006/05/23 09:00
MHDA- 2006/11/11 09:00
CRDT- 2006/05/23 09:00
PHST- 2006/01/02 00:00 [received]
PHST- 2006/03/11 00:00 [revised]
PHST- 2006/03/27 00:00 [accepted]
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/11/11 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - S0014-4835(06)00203-X [pii]
AID - 10.1016/j.exer.2006.03.018 [doi]
PST - ppublish
SO  - Exp Eye Res. 2006 Oct;83(4):783-92. doi: 10.1016/j.exer.2006.03.018. Epub 2006 
      May 19.