PMID- 16713603
OWN - NLM
STAT- MEDLINE
DCOM- 20061027
LR  - 20171116
IS  - 0022-2828 (Print)
IS  - 0022-2828 (Linking)
VI  - 41
IP  - 1
DP  - 2006 Jul
TI  - Salvianolic acid B from Salvia miltiorrhiza inhibits tumor necrosis factor-alpha 
      (TNF-alpha)-induced MMP-2 upregulation in human aortic smooth muscle cells via 
      suppression of NAD(P)H oxidase-derived reactive oxygen species.
PG  - 138-48
AB  - Activated matrix metalloproteinases (MMPs) in patients with acute coronary 
      syndromes may contribute to plaque destabilization. Tumor necrosis factor-alpha 
      (TNF-alpha) enhances NAD (P) H oxidase-dependent reactive oxygen species (ROS) 
      formation and ROS induce MMP-2. In the present study, the effects of a potent 
      water-soluble antioxidant, salvianolic acid B (SalB), derived from a Chinese 
      herb, Salvia miltiorrhiza, on the expression of MMP-2 by TNF-alpha-treated human 
      aortic smooth muscle cells (HASMCs) were investigated. In this study, salvianolic 
      acid B scavenged H2O2 in a dose-dependent manner in test tube. We found that 
      SalB, as well as NADPH oxidase inhibitors, DPI or apocynin, and antioxidant NAC, 
      inhibited TNF-alpha-induced MMP-2 mRNA, protein expression, and gelatinolytic 
      activity in HASMCs in a concentration-dependent manner. We also observed a 
      dose-dependent decrease in ROS production and NADPH oxidase activity induced by 
      TNF-alpha in the presence of SalB. SalB also significantly inhibited angiotensin 
      II or H2O2-induced MMP-2 mRNA and protein expression and gelatinolytic activity 
      in HASMCs. Our data point out that the importance of NADPH oxidase-dependent ROS 
      generation in the control of SalB inhibition of TNF-alpha-induced MMP-2 
      expression and activity.
FAU - Zhang, Hong-Sheng
AU  - Zhang HS
AD  - Department of Biotechnology, Beijing Institute of Radiation Medicine, Taiping 
      Road 27#, Haidian District, Beijing 100850, China.
FAU - Wang, Sheng-Qi
AU  - Wang SQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060519
PL  - England
TA  - J Mol Cell Cardiol
JT  - Journal of molecular and cellular cardiology
JID - 0262322
RN  - 0 (Acetophenones)
RN  - 0 (Benzofurans)
RN  - 0 (Onium Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11128-99-7 (Angiotensin II)
RN  - 6HJ411TU98 (diphenyleneiodonium)
RN  - B6J7B9UDTR (acetovanillone)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - C1GQ844199 (salvianolic acid B)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetophenones/pharmacology
MH  - Acetylcysteine/pharmacology
MH  - Angiotensin II/metabolism/pharmacology
MH  - Aorta/cytology
MH  - Benzofurans/*pharmacology
MH  - Cells, Cultured
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Matrix Metalloproteinase 2/drug effects/genetics/*metabolism
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - NADPH Oxidases/drug effects/metabolism
MH  - Onium Compounds/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Salvia miltiorrhiza/chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism/*pharmacology
MH  - Up-Regulation
EDAT- 2006/05/23 09:00
MHDA- 2006/10/28 09:00
CRDT- 2006/05/23 09:00
PHST- 2005/12/08 00:00 [received]
PHST- 2006/01/22 00:00 [revised]
PHST- 2006/03/14 00:00 [accepted]
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/10/28 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - S0022-2828(06)00079-4 [pii]
AID - 10.1016/j.yjmcc.2006.03.007 [doi]
PST - ppublish
SO  - J Mol Cell Cardiol. 2006 Jul;41(1):138-48. doi: 10.1016/j.yjmcc.2006.03.007. Epub 
      2006 May 19.