PMID- 16714024
OWN - NLM
STAT- MEDLINE
DCOM- 20060925
LR  - 20090115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1123
IP  - 1
DP  - 2006 Aug 4
TI  - Characterization and quantification of the hydrocarbons fraction of the 
      subcutaneous fresh fat of Iberian pig by off-line combination of high performance 
      liquid chromatography and gas chromatography.
PG  - 82-91
AB  - Hydrocarbons in fresh subcutaneous fat of Iberian pig have been analyzed by GC-MS 
      after fractionation of the unsaponifiable fraction with a new off-line 
      combination of HPLC and GC method. The new method proposed improves the recovery 
      and simultaneous quantification of terpenic hydrocarbons in comparison to the 
      traditional LC method. When necessary and for identification purposes, selective 
      ion monitoring (SIM) was used as acquisition mode in GC-MS. To determine the 
      position of the double bonds in the unsaturated hydrocarbon chain the dimethyl 
      disulfide derivatives (DMDS) were obtained. To elucidate the structure of the 
      branched 1-alkenes the hydrocarbon fraction was submitted to hydrogenation. 
      Thirty-five compounds have been identified, including n-alkanes, n-alkenes, 
      branched (n-1,n-2-dimethyl-1-alkenes) and terpenic hydrocarbons, being the most 
      abundant n-alkenes and n-alkanes of even chain of n-C12-n-C26. Besides the 
      hydrocarbons already described in bibliography, a new diterpenic hydrocarbon, 
      ent-kaurene, have been identified for the first time. The compound reported as 
      Neophytadiene by other authors, has been identified as a 20 atoms hydrocarbon 
      with two double bonds, the 7,11,15-trimethyl-heptadeca-1,4-diene.
FAU - Gamero-Pasadas, A
AU  - Gamero-Pasadas A
AD  - Department of Analysis, Instituto de la Grasa (C.S.I.C.), Av. Padre Garcia 
      Tejero, 4 41012 Seville, Spain.
FAU - Viera Alcaide, I
AU  - Viera Alcaide I
FAU - Rios, J J
AU  - Rios JJ
FAU - Graciani Constante, E
AU  - Graciani Constante E
FAU - Vicario, I M
AU  - Vicario IM
FAU - Leon-Camacho, M
AU  - Leon-Camacho M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060522
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Hydrocarbons)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hydrocarbons/*analysis
MH  - Reproducibility of Results
MH  - Subcutaneous Fat/*chemistry
MH  - Swine
EDAT- 2006/05/23 09:00
MHDA- 2006/09/26 09:00
CRDT- 2006/05/23 09:00
PHST- 2006/03/06 00:00 [received]
PHST- 2006/04/25 00:00 [revised]
PHST- 2006/04/28 00:00 [accepted]
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/09/26 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - S0021-9673(06)00906-X [pii]
AID - 10.1016/j.chroma.2006.04.081 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Aug 4;1123(1):82-91. doi: 10.1016/j.chroma.2006.04.081. Epub 
      2006 May 22.