PMID- 16714155
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20131121
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 842
IP  - 2
DP  - 2006 Oct 2
TI  - Sensitive, rapid and validated gas chromatography/negative ion chemical 
      ionization-mass spectrometry assay including derivatisation with a novel chiral 
      agent for the enantioselective quantification of amphetamine-type stimulants in 
      hair.
PG  - 98-105
AB  - A novel chiral derivatisation agent, 
      (2S,4R)-N-heptafluorobutyryl-4-heptafluorobutoyloxy-prolyl chloride, was used for 
      the indirect resolution of amphetamine (AM), methamphetamine (MA), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and 
      3,4-methylenedioxyethylamphetamine (MDEA) enantiomers using gas chromatography 
      coupled to mass spectrometry operating in the negative-ion chemical ionization 
      mode (GC/MS-NICI). This new chiral derivatisation reagent was readily obtained in 
      optically pure form after a simple two-step synthesis. Optimal derivatisation was 
      accomplished in 15 min at room temperature in a carbonate buffer and the 
      resulting diastereoisomers were base line separated by GC in 12 min only. No 
      racemization was observed during the derivatisation. The method was applied and 
      fully validated for the enantiomeric quantification of amphetamines and 
      methylenedioxylated amphetamines in hair. The analyses of 24 hair specimens from 
      suspected ATS abusers showed that 24 cases were positive for MA and/or AM 
      enantiomers and that in most cases the concentrations of (S)-MA and (S)-AM 
      exceeded those of the corresponding (R)-enantiomers. One hair specimen was tested 
      positive for both enantiomers of MDMA and MDA.
FAU - Martins, Liliane Ferreira
AU  - Martins LF
AD  - National Laboratory of Health, Toxicology Division, University of Luxembourg, 
      162A avenue de la Faiencerie, L-1511 Luxembourg, Luxembourg. 
      liliane.martins@uni.lu
FAU - Yegles, Michel
AU  - Yegles M
FAU - Chung, Heesun
AU  - Chung H
FAU - Wennig, Robert
AU  - Wennig R
LA  - eng
PT  - Journal Article
DEP - 20060522
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Amphetamines)
RN  - 44RAL3456C (Methamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Amphetamines/analysis/*chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - Methamphetamine/analysis/chemistry
MH  - Molecular Structure
MH  - N-Methyl-3,4-methylenedioxyamphetamine/analysis/chemistry
MH  - Reproducibility of Results
MH  - Stereoisomerism
EDAT- 2006/05/23 09:00
MHDA- 2006/12/23 09:00
CRDT- 2006/05/23 09:00
PHST- 2005/12/16 00:00 [received]
PHST- 2006/04/12 00:00 [revised]
PHST- 2006/04/14 00:00 [accepted]
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
AID - S1570-0232(06)00348-5 [pii]
AID - 10.1016/j.jchromb.2006.04.024 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Oct 2;842(2):98-105. doi: 
      10.1016/j.jchromb.2006.04.024. Epub 2006 May 22.