PMID- 16714538
OWN - NLM
STAT- MEDLINE
DCOM- 20060615
LR  - 20211216
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 74
IP  - 6
DP  - 2006 Jun
TI  - Significance of heat-stable and heat-labile enterotoxins in porcine 
      colibacillosis in an additive model for pathogenicity studies.
PG  - 3107-14
AB  - Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by 
      enterotoxigenic Escherichia coli (ETEC) have been documented as important factors 
      associated with diarrheal diseases, investigations assessing the contributions of 
      individual enterotoxins to the pathogenesis of E. coli infection have been 
      limited. To address the individual roles of enterotoxins in the diarrheal disease 
      caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative 
      isogenic E. coli strains were constructed by using pBR322 to clone and express LT 
      and STb. Four strains, K88+ astA, K88+ astA/pBR322, K88+ astA STb+, and K88+ astA 
      LT+, were constructed and subsequently included in gnotobiotic piglet challenge 
      studies, and their pathogenesis was assessed. The results indicated that all K88+ 
      isogenic strains were able to colonize the small intestines of piglets exhibiting 
      the K88 receptor. However, only LT- and STb-positive strains caused appreciable 
      diarrhea. Piglets inoculated with the K88+ astA LT+ strain became dehydrated 
      within 18 h, while those inoculated with the K88+ astA STb+ strain did not, 
      although diarrhea developed in several piglets. The changes in the blood 
      packed-cell volume and plasma total protein of gnotobiotic piglets inoculated 
      with the LT-positive strains were significantly greater than those of pigs 
      inoculated with the K88 astA/pBR322 strain (P = 0.012, P = 0.002). 
      Immunochemistry image analysis also suggested that LT enhanced bacterial 
      colonization in a gnotobiotic piglet model. This investigation suggested that LT 
      is a major contributor to the virulence of K88+ ETEC and that isogenic constructs 
      are a useful tool for studying the pathogenesis of ETEC infection.
FAU - Zhang, Weiping
AU  - Zhang W
AD  - Veterinary Science Department, Box 2157, South Dakota State University, 
      Brookings, SD 57006, USA.
FAU - Berberov, Emil M
AU  - Berberov EM
FAU - Freeling, Jessica
AU  - Freeling J
FAU - He, D
AU  - He D
FAU - Moxley, Rodney A
AU  - Moxley RA
FAU - Francis, David H
AU  - Francis DH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/*toxicity
MH  - Diarrhea/etiology
MH  - Enterotoxins/*toxicity
MH  - Escherichia coli Infections/*etiology
MH  - Escherichia coli Proteins/*toxicity
MH  - Intestines/microbiology
MH  - Swine
PMC - PMC1479275
EDAT- 2006/05/23 09:00
MHDA- 2006/06/16 09:00
PMCR- 2006/10/01
CRDT- 2006/05/23 09:00
PHST- 2006/05/23 09:00 [pubmed]
PHST- 2006/06/16 09:00 [medline]
PHST- 2006/05/23 09:00 [entrez]
PHST- 2006/10/01 00:00 [pmc-release]
AID - 74/6/3107 [pii]
AID - 1338-05 [pii]
AID - 10.1128/IAI.01338-05 [doi]
PST - ppublish
SO  - Infect Immun. 2006 Jun;74(6):3107-14. doi: 10.1128/IAI.01338-05.