PMID- 16717426
OWN - NLM
STAT- MEDLINE
DCOM- 20060906
LR  - 20221207
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 70
IP  - 5
DP  - 2006 May
TI  - Reduction of allergenic proteins by the effect of the ripening inhibitor (rin) 
      mutant gene in an F1 hybrid of the rin mutant tomato.
PG  - 1227-33
AB  - The ripening inhibitor (rin) mutant tomato yields non-ripening fruit, and the rin 
      hybrid fruit (RIN/rin) shows an intermediate phenotype between the wild and 
      mutant fruit, that is, red-ripe and extended shelf life. We found by a microarray 
      analysis that the genes encoding possible allergenic proteins were expressed at a 
      significantly lower level in the rin hybrid fruit than in the wild-type fruit. 
      These allergenic proteins, which were beta-fructofuranosidase and 
      polygalacturonase 2A (PG-2A), were confirmed to accumulate at a lower level in 
      the rin hybrid fruit than in the wild-type fruit. The immunoglobulin E (IgE) in 
      serum from a tomato-allergic patient showed lower reactivity to the extract of 
      the rin hybrid fruit than to that of the wild fruit. These results suggest that 
      the rin gene has the potential to regulate allergen accumulation in tomato fruit.
FAU - Kitagawa, Mamiko
AU  - Kitagawa M
AD  - Research Institute, Kagome Co., Ltd. Tochigi, Japan.
FAU - Moriyama, Tatsuya
AU  - Moriyama T
FAU - Ito, Hirotaka
AU  - Ito H
FAU - Ozasa, Sayaka
AU  - Ozasa S
FAU - Adachi, Atsuko
AU  - Adachi A
FAU - Yasuda, Junichi
AU  - Yasuda J
FAU - Ookura, Tetsuya
AU  - Ookura T
FAU - Inakuma, Takahiro
AU  - Inakuma T
FAU - Kasumi, Takafumi
AU  - Kasumi T
FAU - Ishiguro, Yukio
AU  - Ishiguro Y
FAU - Ito, Yasuhiro
AU  - Ito Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Antigens, Plant)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.15 (Polygalacturonase)
RN  - EC 3.2.1.26 (beta-Fructofuranosidase)
SB  - IM
MH  - Antigens, Plant/*genetics/*immunology
MH  - Chimera/genetics
MH  - Food Hypersensitivity/*immunology
MH  - Gene Expression
MH  - Genes, Plant
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Solanum lycopersicum/*adverse effects/genetics/immunology
MH  - Mutation
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Proteins/*genetics/*immunology
MH  - Polygalacturonase/genetics/immunology
MH  - beta-Fructofuranosidase/genetics/immunology
EDAT- 2006/05/24 09:00
MHDA- 2006/09/07 09:00
CRDT- 2006/05/24 09:00
PHST- 2006/05/24 09:00 [pubmed]
PHST- 2006/09/07 09:00 [medline]
PHST- 2006/05/24 09:00 [entrez]
AID - JST.JSTAGE/bbb/70.1227 [pii]
AID - 10.1271/bbb.70.1227 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2006 May;70(5):1227-33. doi: 10.1271/bbb.70.1227.