PMID- 16719359
OWN - NLM
STAT- MEDLINE
DCOM- 20060822
LR  - 20060524
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 319
DP  - 2006
TI  - New approaches to fluorescence in situ hybridization.
PG  - 237-59
AB  - Fluorescence in situ hybridization (FISH) is a nonisotopic labeling and detection 
      method that provides a direct way to determine the relative location or copy 
      number of specific DNA sequences in nuclei or chromosomes. With recent 
      advancements, this technique has found increased application in a number of 
      research areas, including cytogenetics, prenatal diagnosis, cancer research and 
      diagnosis, nuclear organization, gene loss and/or amplification, and gene 
      mapping. The availability of different types of probe and the increasing number 
      of FISH techniques has made it a widespread and diversely applied technology. 
      Multicolor karyotyping by multicolor FISH and spectral karyotyping interphase 
      FISH and comparative genomic hybridization allow genetic analysis of previously 
      intractable targets. We present a brief overview of FISH technology and describe 
      in detail methods of probe labeling and detection for different types of tissue 
      sample, including microdissected nuclei from formalin-fixed paraffin-embedded 
      tissue sections.
FAU - Murthy, Sabita K
AU  - Murthy SK
AD  - Medical Genetics, Al Wasl Hospital, Dubai, United Arab Emirates.
FAU - Demetrick, Douglas J
AU  - Demetrick DJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Cell Cycle
MH  - Chromosomes
MH  - Fluorescent Dyes/*metabolism
MH  - Humans
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Karyotyping/methods
MH  - Neoplasms/genetics/pathology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Prenatal Diagnosis/methods
MH  - Telomere/metabolism
EDAT- 2006/05/25 09:00
MHDA- 2006/08/23 09:00
CRDT- 2006/05/25 09:00
PHST- 2006/05/25 09:00 [pubmed]
PHST- 2006/08/23 09:00 [medline]
PHST- 2006/05/25 09:00 [entrez]
AID - 10.1007/978-1-59259-993-6_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;319:237-59. doi: 10.1007/978-1-59259-993-6_12.