PMID- 16720592
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20151119
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 21
IP  - 9
DP  - 2006 Sep
TI  - Does the ID-MS traceable MDRD equation work and is it suitable for use with 
      compensated Jaffe and enzymatic creatinine assays?
PG  - 2439-45
AB  - BACKGROUND: International recommendations suggest that measurement of serum 
      creatinine should be supplemented with an estimate of glomerular filtration rate 
      (GFR) using the Modification of Diet in Renal Disease (MDRD) study equation. One 
      problem has been the lack of standardization of commercially available creatinine 
      assays resulting in varying estimates of GFR. A revision of the MDRD equation 
      offers traceability to a reference method. This study evaluates the use of 
      isotope dilution mass spectrometry (ID-MS), the compensated Jaffe and enzymatic 
      creatinine methods compared with the Beckman CX3 Jaffe assay used to derive the 
      MDRD equation and investigates their impact on GFR estimation using both the 
      original and ID-MS-traceable MDRD equations. METHODS: Serum creatinine was 
      measured in 277 patients by (i) ID-MS, (ii) a Roche enzymatic assay, (iii) a 
      Roche compensated kinetic Jaffe assay and (iv) a Beckman CX3 kinetic Jaffe assay. 
      Estimated GFR was calculated using the MDRD equations. RESULTS: The ID-MS 
      (-7.5%), Roche enzymatic (-8.6%) and compensated kinetic Jaffe (-11.9%) assays 
      were all negatively biased (P < 0.0001) compared with the Beckman CX3 assay, 
      causing predictable, clinically significant, overestimation of GFR when the 
      original MDRD equation is used. This positive bias was reduced (ID-MS 6.7 to 
      0.4%; enzymatic 8.8 to 3.4%; compensated kinetic Jaffe 13.7 to 7.1%) when GFRs 
      were calculated using the ID-MS-traceable MDRD equation. CONCLUSIONS: Compensated 
      assays that account for non-creatinine chromogen interference produce 
      significantly higher estimates of GFR when using the original MDRD equation. Use 
      of the ID-MS-traceable MDRD equation ameliorates this effect. There is good 
      agreement between estimated GFR derived from the original MDRD equation using 
      Beckman Astra CX3 data and estimated GFR derived from the new ID-MS-traceable 
      MDRD equation using a local ID-MS creatinine assay. This suggests that the 
      ID-MS-traceable MDRD equation may be reliably used with both ID-MS and true 
      ID-MS-traceable creatinine assays without the requirement for standardization to 
      the MDRD laboratory.
FAU - Vickery, Susan
AU  - Vickery S
AD  - Department of Clinical Biochemistry, East Kent Hospitals NHS Trust, Kent and 
      Canterbury Hospital, Canterbury, Kent CT1 3NG, UK. susan.vickery@ekht.nhs.uk
FAU - Stevens, Paul E
AU  - Stevens PE
FAU - Dalton, R Neil
AU  - Dalton RN
FAU - van Lente, Frederick
AU  - van Lente F
FAU - Lamb, Edmund J
AU  - Lamb EJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060523
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Biomarkers/blood
MH  - Calibration
MH  - Creatinine/*blood
MH  - Glomerular Filtration Rate/*physiology
MH  - Humans
MH  - Mass Spectrometry/methods
MH  - Renal Insufficiency, Chronic/*blood/physiopathology
MH  - Reproducibility of Results
EDAT- 2006/05/25 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/05/25 09:00
PHST- 2006/05/25 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/05/25 09:00 [entrez]
AID - gfl249 [pii]
AID - 10.1093/ndt/gfl249 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2006 Sep;21(9):2439-45. doi: 10.1093/ndt/gfl249. Epub 
      2006 May 23.