PMID- 1672122 OWN - NLM STAT- MEDLINE DCOM- 19910412 LR - 20190902 IS - 0198-8859 (Print) IS - 0198-8859 (Linking) VI - 30 IP - 1 DP - 1991 Jan TI - RFLP analysis of the rhesus monkey MHC class II DR subregion. PG - 11-7 AB - Restriction fragment length polymorphism (RFLP) analysis was performed on a panel of 39 serologically typed DR homozygous monkeys. DNA was digested with the restriction enzyme TaqI and hybridizations were carried out with a human leukocyte antigen (HLA)-DR beta 3'UT-specific probe. In addition a panel of 18 monkeys was analyzed comprising experimental autoimmune encephalomyelitis (EAE) susceptible and nonsusceptible animals. The number of DRB/TaqI fragments detected for the various DR specificities varied from two to six, suggesting that the number of DRB genes per haplotype is not constant. RFLP typing allows that most serologically defined DR specificities can be subdivided. This knowledge was applied to define the DR specificities of the animals used for EAE experiments. FAU - Slierendregt, B L AU - Slierendregt BL AD - ITRI-TNO, Primate Center, Rijswijk, The Netherlands. FAU - Otting, N AU - Otting N FAU - Jonker, M AU - Jonker M FAU - Bontrop, R E AU - Bontrop RE LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Hum Immunol JT - Human immunology JID - 8010936 RN - 0 (Histocompatibility Antigens Class II) SB - IM MH - Animals MH - Genes, MHC Class II MH - Haplotypes MH - Histocompatibility Antigens Class II/*genetics MH - Macaca mulatta/genetics/*immunology MH - Polymorphism, Restriction Fragment Length EDAT- 1991/01/01 00:00 MHDA- 1991/01/01 00:01 CRDT- 1991/01/01 00:00 PHST- 1991/01/01 00:00 [pubmed] PHST- 1991/01/01 00:01 [medline] PHST- 1991/01/01 00:00 [entrez] AID - 0198-8859(91)90064-G [pii] AID - 10.1016/0198-8859(91)90064-g [doi] PST - ppublish SO - Hum Immunol. 1991 Jan;30(1):11-7. doi: 10.1016/0198-8859(91)90064-g.