PMID- 1672124
OWN - NLM
STAT- MEDLINE
DCOM- 19910412
LR  - 20190902
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 30
IP  - 1
DP  - 1991 Jan
TI  - DNA amplification for DQ typing as an adjunct to serological prenatal HLA typing 
      for the identification of potential donors for umbilical cord blood 
      transplantation.
PG  - 45-9
AB  - It has recently been demonstrated that umbilical cord blood from genotypically 
      human leukocyte antigen (HLA)-matched donors can provide sufficient numbers of 
      progenitor cells for hematopoietic reconstitution. This technique has been 
      successfully applied in the treatment of two children affected with Fanconi 
      anemia (FA). Fetal cells from the potential sibling donors were first tested to 
      determine that the fetus was not affected with FA. Unaffected fetal cells were 
      then tested for HLA type. Cord blood from compatible donors can be harvested at 
      birth and used immediately or frozen for subsequent use in hematopoietic 
      reconstitution. We now show that fetal cell DNA amplification and hybridization 
      for DQ typing can be an important adjunct procedure to verify serologically 
      determined HLA class II types and/or to establish class II haplotype identity 
      with the affected sibling.
FAU - Pollack, M S
AU  - Pollack MS
AD  - Methodist Hospital, Houston, TX 77030.
FAU - Auerbach, A D
AU  - Auerbach AD
FAU - Broxmeyer, H E
AU  - Broxmeyer HE
FAU - Zaafran, A
AU  - Zaafran A
FAU - Griffith, R L
AU  - Griffith RL
FAU - Erlich, H A
AU  - Erlich HA
LA  - eng
GR  - CA36464/CA/NCI NIH HHS/United States
GR  - CA40552/CA/NCI NIH HHS/United States
GR  - HL32987/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DQ Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Blood Transfusion
MH  - DNA/genetics
MH  - Fanconi Anemia/diagnosis/immunology/therapy
MH  - Female
MH  - Fetal Blood/*cytology/immunology
MH  - *HLA-DQ Antigens/genetics
MH  - Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Infant, Newborn
MH  - Male
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Prenatal Diagnosis
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0198-8859(91)90070-P [pii]
AID - 10.1016/0198-8859(91)90070-p [doi]
PST - ppublish
SO  - Hum Immunol. 1991 Jan;30(1):45-9. doi: 10.1016/0198-8859(91)90070-p.