PMID- 16721606
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20220409
IS  - 0014-4819 (Print)
IS  - 0014-4819 (Linking)
VI  - 175
IP  - 1
DP  - 2006 Oct
TI  - Glyceraldehyde-3-phosphate dehydrogenase versus toluidine blue as a marker for 
      infarct volume estimation following permanent middle cerebral artery occlusion in 
      mice.
PG  - 60-7
AB  - Infarct size is a good predictor of the neurological outcome following stroke. 
      Estimation of infarct size in the early phase following experimental stroke 
      depends on the availability of reliable techniques that can distinguish ischemic 
      from nonischemic tissue. The objective of this study was to provide a simple and 
      robust method for reliable delineation of the ischemic infarct area in fresh 
      frozen cryosections from mice subjected to focal cerebral ischemia. Mice were 
      subjected to permanent middle cerebral artery (MCA) occlusion and euthanised 
      after 30 min, 1, 2, 4, 6, 12 and 24 h. The size of the developing infarct was 
      compared in parallel series of sections in situ hybridized for mRNA encoding the 
      enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or stained with toluidine 
      blue (TB). The infarct was clearly delineated in GAPDH mRNA in situ hybridized 
      sections as soon as 4 h after MCA occlusion. Infarct size was similar at 4 and 6 
      h in GAPDH mRNA in situ hybridized sections. Sections hybridized for GAPDH mRNA 
      showed significantly larger infarcts than sections stained with TB after 6 h but 
      not after 24 h of ischemia. Analysis of in situ hybridized sections revealed 
      changes in neuronal GAPDH mRNA in areas prone to undergo degeneration 30 min to 1 
      h after MCA occlusion, thereby preceding visible pycnosis in TB-stained sections. 
      The results showed that in situ hybridization for GAPDH mRNA was a reliable 
      method and superior to TB staining for precise infarct delineation prior to 6 h 
      of permanent MCA occlusion.
FAU - Clausen, Bettina H
AU  - Clausen BH
AD  - Medical Biotechnology Center, University of Southern Denmark Odense, 
      Winsloewparken 25, Odense C, Denmark. bclausen@health.sdu.dk
FAU - Lambertsen, Kate L
AU  - Lambertsen KL
FAU - Finsen, Bente
AU  - Finsen B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060524
PL  - Germany
TA  - Exp Brain Res
JT  - Experimental brain research
JID - 0043312
RN  - 15XUH0X66N (Tolonium Chloride)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - *Brain Infarction/etiology/metabolism/pathology
MH  - Gene Expression Regulation/physiology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/*metabolism
MH  - In Situ Hybridization/methods
MH  - Infarction, Middle Cerebral Artery/*complications/pathology
MH  - Male
MH  - Mice
MH  - Statistics, Nonparametric
MH  - Time Factors
MH  - *Tolonium Chloride
EDAT- 2006/05/25 09:00
MHDA- 2007/10/03 09:00
CRDT- 2006/05/25 09:00
PHST- 2006/01/02 00:00 [received]
PHST- 2006/04/24 00:00 [accepted]
PHST- 2006/05/25 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2006/05/25 09:00 [entrez]
AID - 10.1007/s00221-006-0526-3 [doi]
PST - ppublish
SO  - Exp Brain Res. 2006 Oct;175(1):60-7. doi: 10.1007/s00221-006-0526-3. Epub 2006 
      May 24.