PMID- 16723531
OWN - NLM
STAT- MEDLINE
DCOM- 20060608
LR  - 20220330
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 26
IP  - 21
DP  - 2006 May 24
TI  - BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic 
      differentiation.
PG  - 5739-49
AB  - In skeletal muscle, brain-derived neurotrophic factor (BDNF) has long been 
      thought to serve as a retrograde trophic factor for innervating motor neurons 
      throughout their lifespan. However, its localization in mature muscle fibers has 
      remained elusive. Given the postulated roles of BDNF in skeletal muscle, we 
      performed a series of complementary experiments aimed at defining the 
      localization of BDNF and its transcripts in adult muscle. By reverse 
      transcription-PCR, in situ hybridization, and immunofluorescence, we show that 
      BDNF, along with the receptor p75NTR, is not expressed at significant levels 
      within mature myofibers and that it does not accumulate preferentially within 
      subsynaptic regions of neuromuscular junctions. Interestingly, expression of BDNF 
      correlated with that of Pax3, a marker of muscle progenitor cells, in several 
      different adult skeletal muscles. Additionally, BDNF was expressed in Pax7+ 
      satellite cells where it colocalized with p75NTR. In complementary cell culture 
      experiments, we detected high levels of BDNF and p75NTR in myoblasts. During 
      myogenic differentiation, expression of BDNF became drastically reduced. Using 
      small interfering RNA (siRNA) technology to knock down BDNF expression, we 
      demonstrate enhanced myogenic differentiation of myoblasts. This accelerated rate 
      of myogenic differentiation seen in myoblasts expressing BDNF siRNA was 
      normalized by administration of recombinant BDNF. Collectively, these findings 
      show that BDNF plays an important regulatory function during myogenic 
      differentiation. In addition, the expression of BDNF in satellite cells is 
      coherent with the notion that BDNF serves a key role in maintaining the 
      population of muscle progenitors in adult muscle.
FAU - Mousavi, Kambiz
AU  - Mousavi K
AD  - Department of Cellular and Molecular Medicine, Center for Neuromuscular Disease, 
      Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1H 8M5, Canada.
FAU - Jasmin, Bernard J
AU  - Jasmin BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Muscle, Skeletal/*cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Nerve Growth Factor/*metabolism
MH  - Satellite Cells, Skeletal Muscle/*cytology/*metabolism
MH  - Tissue Distribution
PMC - PMC6675269
EDAT- 2006/05/26 09:00
MHDA- 2006/06/09 09:00
PMCR- 2006/11/24
CRDT- 2006/05/26 09:00
PHST- 2006/05/26 09:00 [pubmed]
PHST- 2006/06/09 09:00 [medline]
PHST- 2006/05/26 09:00 [entrez]
PHST- 2006/11/24 00:00 [pmc-release]
AID - 26/21/5739 [pii]
AID - 10.1523/JNEUROSCI.5398-05.2006 [doi]
PST - ppublish
SO  - J Neurosci. 2006 May 24;26(21):5739-49. doi: 10.1523/JNEUROSCI.5398-05.2006.