PMID- 16723736
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20191210
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 119
IP  - Pt 11
DP  - 2006 Jun 1
TI  - Caveolin-1 is required for signaling and membrane targeting of EphB1 receptor 
      tyrosine kinase.
PG  - 2299-309
AB  - Eph receptor tyrosine kinases are key players during the development of the 
      embryonic vasculature; however, their role and regulation in adult angiogenesis 
      remain to be defined. Caveolae are flask-shaped invaginations of the cell 
      membrane; their major structural protein, caveolin-1, has been shown to regulate 
      signaling molecules localized in these micro-domains. The interaction of 
      caveolin-1 with several of these proteins is mediated by the binding of its 
      scaffolding domain to a region containing hydrophobic amino acids within these 
      proteins. The presence of such a motif within the EphB1 kinase domain prompted us 
      to investigate the caveolar localization and regulation of EphB1 by caveolin-1. 
      We report that EphB1 receptors are localized in caveolae, and directly interact 
      with caveolin-1 upon ligand stimulation. This interaction, as well as 
      EphB1-mediated activation of extracellular-signal-regulated kinase (ERK), was 
      abrogated by overexpression of a caveolin-1 mutant lacking a functional 
      scaffolding domain. Interaction between Ephs and caveolin-1 is not restricted to 
      the B-subclass of receptors, since we show that EphA2 also interacts with 
      caveolin-1. Furthermore, we demonstrate that the caveolin-binding motif within 
      the kinase domain of EphB1 is primordial for its correct membrane targeting. 
      Taken together, our findings establish caveolin-1 as an important regulator of 
      downstream signaling and membrane targeting of EphB1.
FAU - Vihanto, Meri M
AU  - Vihanto MM
AD  - Department of Nephrology and Hypertension, Inselspital, University of Bern, 
      Switzerland.
FAU - Vindis, Cecile
AU  - Vindis C
FAU - Djonov, Valentin
AU  - Djonov V
FAU - Cerretti, Douglas P
AU  - Cerretti DP
FAU - Huynh-Do, Uyen
AU  - Huynh-Do U
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Caveolin 1)
RN  - 0 (Ephrin-B2)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.7.10.1 (Receptor, EphA2)
RN  - EC 2.7.10.1 (Receptor, EphB1)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Caveolin 1/drug effects/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Membrane/*metabolism
MH  - Chlorocebus aethiops
MH  - Cholesterol/pharmacology
MH  - Cricetinae
MH  - Ephrin-B2/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - Humans
MH  - Phosphorylation
MH  - Protein Transport/physiology
MH  - Receptor, EphA2/metabolism
MH  - Receptor, EphB1/*metabolism
MH  - Signal Transduction/*physiology
EDAT- 2006/05/26 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/05/26 09:00
PHST- 2006/05/26 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/05/26 09:00 [entrez]
AID - 119/11/2299 [pii]
AID - 10.1242/jcs.02946 [doi]
PST - ppublish
SO  - J Cell Sci. 2006 Jun 1;119(Pt 11):2299-309. doi: 10.1242/jcs.02946.