PMID- 167265
OWN - NLM
STAT- MEDLINE
DCOM- 19751011
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 24
IP  - 8
DP  - 1975 Aug
TI  - Effects of insulin, tolbutamide, and glucagon on activities of jejunal 
      carbohydrate-metabolizing enzymes in humans.
PG  - 923-8
AB  - The activities of jejunal carbohydrate-metabolizing enzymes show adaptive drugs, 
      and sex hormones. To learn whether insulin, tolbutamide, and glucagon had effects 
      on these enzymes, we performed serial peroral jejunal biopsies in normal young 
      men and in obese patients, before and after treatment with these agents. Jejunal 
      mucosa was assayed for glycolytic enzyme activities, pyruvate kinase (PK), 
      hexokinase (HK), and fructose-1,6-diphosphate aldolase (FDPA), and the 
      nonglycolytic enzyme activity, fructose diphosphatase (FDPase). Insulin 
      significantly increased the activity of jejunal PK (+48% change from control) and 
      HK (+6%), decreased the activity of FDPase (-36%),and had no effect on FDPA. 
      Glucagon had opposite effects; the activity of PK was decreased (-33%) and FDPase 
      was increased (+50%). Tolbutamide significantly increased the activities of PK 
      (+47%), HK (+14%), and FDPA (+7%), and decreased the activities of FDPase (-36%). 
      The results of tolbutamide on glycolytic enzyme activities were independent of 
      endogenous insulin. The data support the concept that jejunal 
      carbohydrate-metabolizing enzymes in man respond to hormones and drugs similar to 
      responses observed in rat liver. This is important because it now gives us a 
      means of studying the actions of these hormones directly in human tissue.
FAU - Lufkin, E G
AU  - Lufkin EG
FAU - Taunton, O D
AU  - Taunton OD
FAU - Stifel, F B
AU  - Stifel FB
FAU - Rosensweig, N S
AU  - Rosensweig NS
FAU - Hagler, L
AU  - Hagler L
FAU - Teplick, R S
AU  - Teplick RS
FAU - Herman, R H
AU  - Herman RH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Insulin)
RN  - 9007-92-5 (Glucagon)
RN  - 982XCM1FOI (Tolbutamide)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 3.1.3.11 (Fructose-Bisphosphatase)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Carbohydrate Metabolism
MH  - Fructose-Bisphosphatase/metabolism
MH  - Fructose-Bisphosphate Aldolase/metabolism
MH  - Glucagon/*pharmacology
MH  - Glycolysis
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Insulin/*pharmacology
MH  - Jejunum/*enzymology
MH  - Male
MH  - Middle Aged
MH  - Obesity/*enzymology
MH  - Phosphofructokinase-1/metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Species Specificity
MH  - Tolbutamide/*pharmacology
EDAT- 1975/08/01 00:00
MHDA- 1975/08/01 00:01
CRDT- 1975/08/01 00:00
PHST- 1975/08/01 00:00 [pubmed]
PHST- 1975/08/01 00:01 [medline]
PHST- 1975/08/01 00:00 [entrez]
AID - 0026-0495(75)90083-9 [pii]
AID - 10.1016/0026-0495(75)90083-9 [doi]
PST - ppublish
SO  - Metabolism. 1975 Aug;24(8):923-8. doi: 10.1016/0026-0495(75)90083-9.