PMID- 16726929
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20091216
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 35
IP  - 3
DP  - 1991 Mar
TI  - Determination of ovulation time in bitches based on teasing, vaginal cytology, 
      and elisa for progesterone.
PG  - 603-11
AB  - The estrous cycle of 16 mature mongrel female dogs was monitored to evaluate the 
      accuracy of teasing, vaginal cytology and quantitative ELISA progesterone assay 
      to determine ovulation. The dogs were presented to male, and blood samples and 
      vaginal swabs were taken daily during proestrus and estrus. Selected serum 
      samples collected during estrus were assayed for endogenous LH by 
      radioimmunoassay (RIA). Plasma samples collected during proestrus and estrus were 
      assayed for progesterone with a commercially available ELISA kit. Ovulation was 
      considered to take place 48 h after the preovulatory LH peak. Vaginal cytology 
      smears were stained with Wright's stain and evaluated for the percentage of 
      superficial squamous cells. Day 1 of diestrus (Day 1) was defined as a drop of 
      20% or more in the total number of superficial cells. Two standard curves (linear 
      and best fitted curves) commonly used with ELISA were compared together and with 
      the RIA progesterone assay. Ovulation was estimated to occur when progesterone 
      concentration was 4.9 +/- 1.0 ng/ml (mean +/- SD, n = 15), with a range of 3.4 to 
      6.6 ng/ml. Based on vaginal cytology, ovulation took place 6.9 +/- 1.6 d (n = 15) 
      after 80% of the squamous cells were superficial and 6.8 +/- 1.4 d (n = 16) 
      before Day 1. Ovulation took place 2.1 +/- 3.9 d (n=11) after the first day of 
      standing estrus and 8.8 +/- 1.5 d (n = 10) before the last day of receptivity. 
      The two standard curves were found parallel to each other and to the RIA 
      progesterone assay. Based on the results of the present study, ELISA progesterone 
      assay and determination of the first day of estrus by vaginal cytology are 
      reliable methods for predicting ovulation, whereas the last day of receptivity as 
      determined by teasing and Day 1 as determined by vaginal cytology are reliable 
      methods to retrospectively estimate ovulation time.
FAU - Bouchard, G F
AU  - Bouchard GF
AD  - Department of Medicine and Surgery, University of Missouri, Columbia, MO 65211, 
      USA.
FAU - Solorzano, N
AU  - Solorzano N
FAU - Concannon, P W
AU  - Concannon PW
FAU - Youngquist, R S
AU  - Youngquist RS
FAU - Bierschwal, C J
AU  - Bierschwal CJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1990/09/18 00:00 [received]
PHST- 1990/11/27 00:00 [accepted]
PHST- 1990/09/18 00:00 [received]
PHST- 1990/11/27 00:00 [accepted]
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 0093-691X(91)90456-N [pii]
AID - 10.1016/0093-691x(91)90456-n [doi]
PST - ppublish
SO  - Theriogenology. 1991 Mar;35(3):603-11. doi: 10.1016/0093-691x(91)90456-n.