PMID- 16727802
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070717
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 45
IP  - 2
DP  - 1996 Jan 15
TI  - Semen changes in boars after experimental infection with porcine reproductive and 
      respiratory syndrome (PRRS) virus.
PG  - 383-95
AB  - Eleven boars seronegative to porcine reproductive and respiratory syndrome virus 
      (PRRSV) were trained for semen collection: five boars were inoculated 
      intranasally with 6 x 10(6)TCID(50)/ml of PRRSV (Group A); four boars were 
      inoculated intranasally with 6 x 10(4)TCID(50)/ml (Group B); and two boars were 
      used as uninfected control (Group C). Semen samples were collected at 7-d 
      intervals from 49 d prior to experimental inoculation with PRRSV to 70 d after 
      inoculation, and were examined for sperm volume, sperm concentration, sperm 
      morphology, sperm motility and for the presence of PRRSV. The infection in boars 
      was demonstrated by the reisolation of PRRSV from the serum of all inoculated 
      boars. Rectal temperatures and general health of the boars were clinically normal 
      throughout the trial. Differences were observed in the quality of semen collected 
      from boars after experimental infection with PRRSV. This infection induced a 
      significant decrease in sperm motility and in spermatozoa with normal acrosomes. 
      Of the semen samples tested for virus isolation in swine alveolar macrophages 
      PRRSV was only isolated in 1 boar from Group B. The virus was detected in an 
      additional semen sample in Group A by the production of an antibody titer in a 
      biological assay. All attempts to detect PRRSV by RT-PCR in semen samples were 
      unsuccessful. Nevertheless, from our study it is possible to suggest that the 
      PRRSV can occasionally be transmitted in the semen during the initial phase of 
      the disease.
FAU - Prieto, C
AU  - Prieto C
AD  - Departamento de Patologia Animal I. Universidad Complutense de Madrid, Facultad 
      de Veterinaria 28040 Madrid, Spain.
FAU - Suarez, P
AU  - Suarez P
FAU - Bautista, J M
AU  - Bautista JM
FAU - Sanchez, R
AU  - Sanchez R
FAU - Rillo, S M
AU  - Rillo SM
FAU - Simarro, I
AU  - Simarro I
FAU - Solana, A
AU  - Solana A
FAU - Castro, J M
AU  - Castro JM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
EDAT- 1996/01/15 00:00
MHDA- 1996/01/15 00:01
CRDT- 1996/01/15 00:00
PHST- 1994/10/25 00:00 [received]
PHST- 1995/09/21 00:00 [accepted]
PHST- 1994/10/25 00:00 [received]
PHST- 1995/09/21 00:00 [accepted]
PHST- 1996/01/15 00:00 [pubmed]
PHST- 1996/01/15 00:01 [medline]
PHST- 1996/01/15 00:00 [entrez]
AID - 0093-691X(95)00375-I [pii]
AID - 10.1016/0093-691x(95)00375-i [doi]
PST - ppublish
SO  - Theriogenology. 1996 Jan 15;45(2):383-95. doi: 10.1016/0093-691x(95)00375-i.