PMID- 16727934
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070717
LR  - 20060526
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 46
IP  - 4
DP  - 1996 Sep
TI  - Effect of porcine reproductive and respiratory syndrome virus (PRRSV) on 
      development of porcine fertilized ova in vitro.
PG  - 687-93
AB  - Fertilized pig ova, collected from 14 estrus-synchronized gilts 32 h after 
      presumed ovulation were microinjected or cultured for 72 h in Beltsville Embryo 
      Culture Medium-3 with or without Porcine Reproductive and Respiratory Syndrome 
      Virus (PRRSV). To detect virus in the samples, virus isolation of swine alveolar 
      macrophage, Reverse Transcriptase Polymerase Chain Reaction and Fluorescent 
      Antibody techniques were employed. Microinjection or incubation of embryos with 
      PRRSV did not significantly inhibit development of the porcine embryos in vitro 
      when compared with that of controls (P = 0.75 and P = 0.14, respectively). 
      Although either 10 to 20 TCID50 were microinjected or large concentrations of 
      virus were used for embryo exposure by incubation, PRRSV was not detected in 
      association with the embryos. It is concluded based on the experiments reported 
      here that 4- to 16-cell stage pig embryos are not susceptible to productive 
      infection with PRRSV in vitro.
FAU - Prieto, C
AU  - Prieto C
AD  - Departamento de Patologia Animal I, Universidad Complutense de Madrid, Facultad 
      de Veterinaria, 28040 Madrid, Spain.
FAU - Suarez, P
AU  - Suarez P
FAU - Martin-Rillo, S
AU  - Martin-Rillo S
FAU - Simarro, I
AU  - Simarro I
FAU - Solana, A
AU  - Solana A
FAU - Castro, J M
AU  - Castro JM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1995/10/09 00:00 [received]
PHST- 1996/03/07 00:00 [accepted]
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 0093-691X(96)00220-8 [pii]
AID - 10.1016/0093-691X(96)00220-8 [doi]
PST - ppublish
SO  - Theriogenology. 1996 Sep;46(4):687-93. doi: 10.1016/0093-691X(96)00220-8.