PMID- 16728085
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070625
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 47
IP  - 7
DP  - 1997 May
TI  - Susceptibility of zona-intact and zona-free in vitro-produced bovine embryos at 
      different stages of development to infection with bovine herpesvirus-1.
PG  - 1389-402
AB  - The aim of the present study was to determine if BHV-1 is able to replicate 
      within in vitro produced embryos and to investigate the degree to which the zona 
      pellucida (ZP) is able to protect in vitro produced embryos against infection 
      with BHV-1. Both ZP-intact and ZP-free matured oocytes, zygotes (1 d post 
      insemination; 1dpi), 8-cell stage embryos (3 dpi), morulae (6 dpi) were incubated 
      for 1 h in 1 ml of MEM containing 10(7.7) TCID(50)/ml BHV-1 (Cooper strain). 
      Three titers (10(5.7), 10(6.7) and 10(7.7) TCID(50)/ml) of the Cooper strain were 
      used for incubation of hatched blastocysts (9 dpi). Bovine embryonic lung cells 
      (BEL) on microcarriers were inoculated following the same protocol as for the 
      embryos. At 0, 12, 24, 36 and 48 h post inoculation (hpi), groups of embryos and 
      BEL cells were collected for virus titration and for the determination of the 
      percentage of viral antigen positive cells by immunofluorescence. For the 3 
      developmental stages in ZP-free embryos, similar maximal intracellular virus 
      progeny titers were obtained at 24 to 48 hpi ranging from 10(1.32) to 10(1.43) 
      TCID(50)/ 100 embryonic cells. The intracellular virus titer in the BEL cells 
      peaked at 10(3.08) TCID(50)/ 100 BEL cells. The percentage of cells which 
      expressed viral antigens was 13% in ZP-free hatched blastocysts, 17% in ZP-free 
      morulae and 100% in BEL cells. In ZP-intact embryos, no replication of BHV-1 was 
      detected. These results clearly show that only after removal of the zona 
      pellucida, BHV-1 is able to replicate within the in vitro produced embryos, with 
      only a subset of embryonic cells being fully susceptible.
FAU - Vanroose, G
AU  - Vanroose G
AD  - Department of Obstetrics, Reproduction and Herd Health, Brussels, Belgium.
FAU - Nauwynck, H
AU  - Nauwynck H
FAU - Van Soom, A
AU  - Van Soom A
FAU - Vanopdenbosch, E
AU  - Vanopdenbosch E
FAU - de Kruif, A
AU  - de Kruif A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1996/09/16 00:00 [received]
PHST- 1996/12/20 00:00 [accepted]
PHST- 1996/09/16 00:00 [received]
PHST- 1996/12/20 00:00 [accepted]
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0093-691X(97)00130-1 [pii]
AID - 10.1016/s0093-691x(97)00130-1 [doi]
PST - ppublish
SO  - Theriogenology. 1997 May;47(7):1389-402. doi: 10.1016/s0093-691x(97)00130-1.