PMID- 16728150
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070626
LR  - 20190823
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 48
IP  - 4
DP  - 1997 Sep
TI  - Repeated endoscopic ovum pick-up in hormonally untreated ewes: a new technique.
PG  - 545-50
AB  - Multiple ovulation and embryo transfer (MOET) are cumbersome and may not be used 
      in all animals. An alternative method for obtaining embryos is to harvest ova and 
      subject them to in vitro maturation/fertilization (IVM/IVF). We tested a modified 
      endoscopic technique designed to allow repeated recovery of oocytes from donor 
      ewes without any hormonal treatment. Seventeen randomly chosen Merino donors were 
      used for ovum pick-up 5 times (OPU1 to OPU5) at 1-wk intervals. The OPUs were 
      performed by ventral laparoscopy. The follicular fluid was aspirated through a 
      needle (0.9 x 75 mm) connected to a 5 ml-syringe. A total of 385 oocytes was 
      collected from 567 aspirated follicles for a collection rate of 67.9%. The number 
      of follicles and oocytes per ewe and the collection rate did not differ 
      significantly (P > 0.05) between collection periods; however, a large variability 
      in the number of follicles and oocytes was observed among individual animals. The 
      collection rate tended to be higher in donors which showed a higher number of 
      follicles. Possible reasons for this are discussed. Oocytes were divided into 4 
      classes based upon morphological criteria. The portion of oocytes suitable for in 
      vitro production (Classes I to III) were similar among collection periods (83% in 
      OPU1 vs 84% in OPU5). The technique described in this study is useful for 
      obtaining large numbers of oocytes from individual animals during a defined 
      period. This method combined with established in vitro production programs 
      provides a way to increase the number of offspring from genetically valuable 
      animals even when they are affected by infertility due to adhesions or 
      deformations of the oviduct and/or uterus.
FAU - Kuhholzer, B
AU  - Kuhholzer B
AD  - Institute of Animal Breeding and Genetics, University of Veterinary Science, 
      Vienna, Austria.
FAU - Muller, S
AU  - Muller S
FAU - Treuer, A
AU  - Treuer A
FAU - Seregi, J
AU  - Seregi J
FAU - Besenfelder, U
AU  - Besenfelder U
FAU - Brem, G
AU  - Brem G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
EDAT- 2006/05/27 09:00
MHDA- 2006/05/27 09:01
CRDT- 2006/05/27 09:00
PHST- 1996/09/06 00:00 [received]
PHST- 1997/04/08 00:00 [accepted]
PHST- 1996/09/06 00:00 [received]
PHST- 1997/04/08 00:00 [accepted]
PHST- 2006/05/27 09:00 [pubmed]
PHST- 2006/05/27 09:01 [medline]
PHST- 2006/05/27 09:00 [entrez]
AID - S0093-691X(97)00271-9 [pii]
AID - 10.1016/s0093-691x(97)00271-9 [doi]
PST - ppublish
SO  - Theriogenology. 1997 Sep;48(4):545-50. doi: 10.1016/s0093-691x(97)00271-9.