PMID- 16730733
OWN - NLM
STAT- MEDLINE
DCOM- 20070410
LR  - 20181201
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 191
IP  - 1
DP  - 2007 Mar
TI  - Unsaturated fatty acids suppress the expression of the ATP-binding cassette 
      transporter G1 (ABCG1) and ABCA1 genes via an LXR/RXR responsive element.
PG  - 11-21
AB  - ATP-binding cassette transporters (ABC) G1 and ABCA1 are membrane cholesterol 
      transporters and have been implicated to mediate cholesterol efflux from cells in 
      the presence of high density lipoproteins and its major protein constituent 
      apolipoprotein A-I, respectively. We previously demonstrated that unsaturated 
      fatty acids suppress the stimulatory effects of oxysterols and retinoids on ABCA1 
      gene transcription. We here demonstrate that unsaturated fatty acids 
      significantly suppress the stimulatory effects of oxysterols and retinoids on the 
      expression of ABCG1 mRNA and protein and the activity of the wild-type human 
      ABCG1 promoter as well as ABCA1. Mutation or deletion of the DR4 element within 
      the ABCG1 or ABCA1 promoters, to which the transcriptional inducers LXR and RXR 
      bind, abolished the suppressive effects of unsaturated fatty acids. Our 
      observations provide the first evidence that unsaturated fatty acids suppress 
      ABCG1 gene expression by a mechanism which involves the binding of LXR/RXR to the 
      promoters. Suppression of both the ABCA1 and ABCG1 genes may indicate that 
      unsaturated fatty acids suppress not only cholesterol efflux to apoA-I and 
      thereby nascent HDL formation but also HDL-dependent cholesterol efflux from 
      vascular cells.
FAU - Uehara, Yoshinari
AU  - Uehara Y
AD  - Department of Neurology and Healthcare, Fukuoka University Hospital, 7-45-1 
      Nanakuma, Jonan-ku, 814-0180 Fukuoka, Japan. ueharay@fukuoka-u.ac.jp
FAU - Miura, Shin-ichiro
AU  - Miura S
FAU - von Eckardstein, Arnold
AU  - von Eckardstein A
FAU - Abe, Satomi
AU  - Abe S
FAU - Fujii, Akihiro
AU  - Fujii A
FAU - Matsuo, Yoshino
AU  - Matsuo Y
FAU - Rust, Stephan
AU  - Rust S
FAU - Lorkowski, Stefan
AU  - Lorkowski S
FAU - Assmann, Gerd
AU  - Assmann G
FAU - Yamada, Tatsuo
AU  - Yamada T
FAU - Saku, Keijiro
AU  - Saku K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060530
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Lipoproteins)
RN  - 0 (Liver X Receptors)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Retinoid X Receptors)
SB  - IM
MH  - ATP Binding Cassette Transporter 1
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Cholesterol, LDL/metabolism
MH  - DNA-Binding Proteins/physiology
MH  - Fatty Acids, Unsaturated/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Lipoproteins/genetics/*metabolism
MH  - Liver X Receptors
MH  - Mice
MH  - Orphan Nuclear Receptors
MH  - Point Mutation
MH  - Promoter Regions, Genetic/*physiology
MH  - Receptors, Cytoplasmic and Nuclear/physiology
MH  - Retinoid X Receptors/physiology
EDAT- 2006/05/30 09:00
MHDA- 2007/04/11 09:00
CRDT- 2006/05/30 09:00
PHST- 2005/05/06 00:00 [received]
PHST- 2006/04/11 00:00 [revised]
PHST- 2006/04/13 00:00 [accepted]
PHST- 2006/05/30 09:00 [pubmed]
PHST- 2007/04/11 09:00 [medline]
PHST- 2006/05/30 09:00 [entrez]
AID - S0021-9150(06)00212-7 [pii]
AID - 10.1016/j.atherosclerosis.2006.04.018 [doi]
PST - ppublish
SO  - Atherosclerosis. 2007 Mar;191(1):11-21. doi: 
      10.1016/j.atherosclerosis.2006.04.018. Epub 2006 May 30.