PMID- 16731844
OWN - NLM
STAT- MEDLINE
DCOM- 20070723
LR  - 20060529
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 55
IP  - 6
DP  - 2006 Jun
TI  - Downregulation of electron transport chain genes in visceral adipose tissue in 
      type 2 diabetes independent of obesity and possibly involving tumor necrosis 
      factor-alpha.
PG  - 1792-9
AB  - Impaired oxidative phosphorylation is suggested as a factor behind insulin 
      resistance of skeletal muscle in type 2 diabetes. The role of oxidative 
      phosphorylation in adipose tissue was elucidated from results of Affymetrix gene 
      profiling in subcutaneous and visceral adipose tissue of eight nonobese healthy, 
      eight obese healthy, and eight obese type 2 diabetic women. Downregulation of 
      several genes in the electron transport chain was the most prominent finding in 
      visceral fat of type 2 diabetic women independent of obesity, but the gene 
      pattern was distinct from that previously reported in skeletal muscle in type 2 
      diabetes. A similar but much weaker effect was observed in subcutaneous fat. 
      Tumor necrosis factor-alpha (TNF-alpha) is a major factor behind inflammation and 
      insulin resistance in adipose tissue. TNF-alpha treatment decreased mRNA 
      expression of electron transport chain genes and also inhibited fatty acid 
      oxidation when differentiated human preadipocytes were treated with the cytokine 
      for 48 h. Thus, type 2 diabetes is associated with a tissue- and region-specific 
      downregulation of oxidative phosphorylation genes that is independent of obesity 
      and at least in part mediated by TNF-alpha, suggesting that impaired oxidative 
      phosphorylation of visceral adipose tissue has pathogenic importance for 
      development of type 2 diabetes.
FAU - Dahlman, Ingrid
AU  - Dahlman I
AD  - Department of Medicine, Karolinska University Hospital, Huddinge, SE-141 86 
      Stockholm, Sweden.
FAU - Forsgren, Margaretha
AU  - Forsgren M
FAU - Sjogren, Annelie
AU  - Sjogren A
FAU - Nordstrom, Elisabet Arvidsson
AU  - Nordstrom EA
FAU - Kaaman, Maria
AU  - Kaaman M
FAU - Naslund, Erik
AU  - Naslund E
FAU - Attersand, Anneli
AU  - Attersand A
FAU - Arner, Peter
AU  - Arner P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Fatty Acids)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Type 2/*genetics/metabolism
MH  - Down-Regulation/drug effects
MH  - Electron Transport/genetics
MH  - Fatty Acids/metabolism
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Intra-Abdominal Fat/drug effects/*metabolism
MH  - Middle Aged
MH  - Obesity/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Oxidation-Reduction/drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2006/05/30 09:00
MHDA- 2007/07/24 09:00
CRDT- 2006/05/30 09:00
PHST- 2006/05/30 09:00 [pubmed]
PHST- 2007/07/24 09:00 [medline]
PHST- 2006/05/30 09:00 [entrez]
AID - 55/6/1792 [pii]
AID - 10.2337/db05-1421 [doi]
PST - ppublish
SO  - Diabetes. 2006 Jun;55(6):1792-9. doi: 10.2337/db05-1421.