PMID- 16732382
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20061115
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 95
IP  - 6
DP  - 2006 Jun
TI  - Overexpression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in 
      mice does not affect adipogenesis or adipose tissue development.
PG  - 1019-24
AB  - In order to evaluate a potential functional role of tissue inhibitor of matrix 
      metalloproteinases-1 (TIMP-1) in development of obesity, we studied the effect of 
      overexpression of human TIMP-1 (hTIMP-1) in C57Bl/6J mice in vivo and in 
      3T3-F442A preadipocytes in vitro. Stable long-term overexpression of hTIMP-1 in 
      mice was achieved by adenoviral gene transfer, yielding plasma levels exceeding 
      250 ng/ml at eight weeks after injection. Mice overexpressing hTIMP-1 and kept on 
      a high fat diet for 14 weeks had body weights, adipose tissue weights, and 
      adipocyte diameters that were somewhat, but not significantly, lower than those 
      of control mice. Similar observations were made after overexpression of hTIMP-1 
      in mice with lipectomy of the subcutaneous adipose tissue, kept on a high fat 
      diet for 20 weeks. In both in vivo models, blood vessels in the adipose tissues 
      were significantly smaller after hTIMP-1 gene transfer than in control mice. 
      Overexpression of hTIMP-1 in 3T3-F442A preadipocytes had no effect on their 
      subsequent differentiation into mature adipocytes. Thus, overexpression of 
      hTIMP-1 in mice had no significant effect on ongoing adipogenesis or adipose 
      tissue development, although the blood vessel size in adipose tissues was 
      reduced.
FAU - Demeulemeester, Diego
AU  - Demeulemeester D
AD  - Center for Molecular and Vascular Biology, K. U. Leuven, Campus Gasthuisberg, O & 
      N, Herestraat 49, B-3000, Leuven, Belgium.
FAU - Scroyen, Ilse
AU  - Scroyen I
FAU - Voros, Gabor
AU  - Voros G
FAU - Snoeys, Jan
AU  - Snoeys J
FAU - De Geest, Bart
AU  - De Geest B
FAU - Collen, Desire
AU  - Collen D
FAU - Lijnen, H Roger
AU  - Lijnen HR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Dietary Fats)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/*cytology/metabolism
MH  - *Adipogenesis
MH  - Animals
MH  - Blood Vessels/pathology
MH  - Dietary Fats/administration & dosage
MH  - Lipectomy
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Obesity/*metabolism/pathology
MH  - Subcutaneous Fat/blood supply/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/blood/genetics/*metabolism
MH  - Transfection
EDAT- 2006/05/30 09:00
MHDA- 2006/09/22 09:00
CRDT- 2006/05/30 09:00
PHST- 2006/05/30 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/05/30 09:00 [entrez]
AID - 06061019 [pii]
AID - 10.1160/TH05-11-0742 [doi]
PST - ppublish
SO  - Thromb Haemost. 2006 Jun;95(6):1019-24. doi: 10.1160/TH05-11-0742.