PMID- 16732938
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20060530
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 27
IP  - 1
DP  - 2006 Jan
TI  - [Application of dual-color fluorescence in situ hybridization in acute myeloid 
      leukemia with t (8; 21)].
PG  - 32-5
AB  - OBJECTIVE: To investigate the utilities of dual-color fluorescence in situ 
      hybridization (FISH) in diagnosis and monitor of treatment in acute myeloid 
      leukemia (AML) with t (8; 21). METHODS: Seventy patients having FISH results were 
      divided into two groups: untreated and treated group. Comparative analysis was 
      performed between the results of conventional cytogenetic analysis (CCA) and FISH 
      analysis, and in some of them, between FISH and reverse transcriptase polymerase 
      chain reaction (RT-PCR) results. A successive FISH following R-banding was 
      carried out in those with cytogenetic undetermined cases. RESULTS: In untreated 
      group, 30/42 cases of t (8; 21) AML were positive for AML1/ETO in FISH assay. 
      Three cases were positive for AML/ETO by FISH although two of them lacked t (8; 
      21) by CCA and one negative for AML1/ETO by RT-PCR. Six cases with complex 
      karyotype abnormalities were confirmed to be AML1/ETO positive by the successive 
      R-banding and FISH assay, and the involved genes were clearly visualized in FISH 
      image. In the treated group, there were 28 cases of t (8; 21) AML diagnosed. 
      Three cases without t (8; 21) by CCA were positive by FISH. Two patients were 
      detected relapse earlier by FISH. CONCLUSION: The dual-color FISH technique is a 
      much more sensitive and accurate approach to the diagnosis of t (8; 21) AML and 
      minimal residual disease (MRD) monitoring. It can also provide precise mapping of 
      fusion signals in complex karyotype.
FAU - Li, Cheng-wen
AU  - Li CW
AD  - Institute of Hematology and Blood Disease Hospital, CAMS and PUMC, Tianjin 
      300020, China.
FAU - Bo, Li-jin
AU  - Bo LJ
FAU - Dai, Yun
AU  - Dai Y
FAU - Liu, Xu-ping
AU  - Liu XP
FAU - Qin, Shuang
AU  - Qin S
FAU - Liu, Shi-he
AU  - Liu SH
FAU - Wang, Jian-xiang
AU  - Wang JX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 2006/05/31 09:00
MHDA- 2009/10/01 06:00
CRDT- 2006/05/31 09:00
PHST- 2006/05/31 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2006/05/31 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2006 Jan;27(1):32-5.