PMID- 16735376
OWN - NLM
STAT- MEDLINE
DCOM- 20060818
LR  - 20171116
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 18
IP  - 7
DP  - 2006 Jul
TI  - Spontaneous B cell hyperactivity in autoimmune-prone MRL mice.
PG  - 1127-37
AB  - The MRL-lpr/lpr mouse strain is a commonly used model of the human autoimmune 
      disease systemic lupus erythematosus (SLE). Although much is known about the 
      contribution of the lpr Fas mutation to B cell tolerance breakdown, the role of 
      the genetic background of the MRL strain itself is less well explored. In this 
      study, we use the MD4 anti-hen egg lysozyme Ig (IgHEL) transgenic system to 
      explore B cell function in MRL+/+ and non-autoimmune mice. We demonstrate that 
      MRL IgHEL B cells show spontaneous hyperactivity in the absence of self-antigen, 
      which is associated with low total B cell numbers but an expansion of the 
      marginal zone B cell population. However, B cell anergy is normal in the presence 
      of soluble lysozyme [soluble hen egg lysozyme (sHEL)], and MRL IgHEL B cells 
      undergo normal elimination in the presence of sHEL when competing with a 
      polyclonal C57BL/6 B cell repertoire. We conclude that B cell hyperactivity may 
      contribute to the autoimmune phenotype of MRL+/+ and MRL-lpr/lpr strains when it 
      initiates antibody responses to rare or sequestered antigens that are below the 
      threshold for tolerance induction, but that there is no B cell intrinsic defect 
      in anergy in MRL mice.
FAU - Nijnik, Anastasia
AU  - Nijnik A
AD  - Henry Wellcome Building of Molecular Physiology, Oxford University, Roosevelt 
      Drive, Oxford, OX3 7BN, UK.
FAU - Ferry, Helen
AU  - Ferry H
FAU - Lewis, Graham
AU  - Lewis G
FAU - Rapsomaniki, Eleni
AU  - Rapsomaniki E
FAU - Leung, Janson C H
AU  - Leung JC
FAU - Daser, Angelika
AU  - Daser A
FAU - Lambe, Teresa
AU  - Lambe T
FAU - Goodnow, Christopher C
AU  - Goodnow CC
FAU - Cornall, Richard J
AU  - Cornall RJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060530
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (fas Receptor)
SB  - IM
MH  - Animals
MH  - Antibody Formation/genetics/*immunology
MH  - B-Lymphocytes/*immunology/pathology
MH  - Humans
MH  - Lupus Erythematosus, Systemic/genetics/*immunology/pathology
MH  - Lymphocyte Activation/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred MRL lpr
MH  - Mice, Transgenic
MH  - Species Specificity
MH  - fas Receptor/genetics/immunology
EDAT- 2006/06/01 09:00
MHDA- 2006/08/19 09:00
CRDT- 2006/06/01 09:00
PHST- 2006/06/01 09:00 [pubmed]
PHST- 2006/08/19 09:00 [medline]
PHST- 2006/06/01 09:00 [entrez]
AID - dxl047 [pii]
AID - 10.1093/intimm/dxl047 [doi]
PST - ppublish
SO  - Int Immunol. 2006 Jul;18(7):1127-37. doi: 10.1093/intimm/dxl047. Epub 2006 May 
      30.