PMID- 16736504
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20240129
IS  - 0196-8092 (Print)
IS  - 0196-8092 (Linking)
VI  - 38
IP  - 6
DP  - 2006 Jul
TI  - Time-domain and spectral-domain optical coherence tomography in the analysis of 
      brain tumor tissue.
PG  - 588-97
AB  - INTRODUCTION: Detection of residual tumor during resection of glial brain tumors 
      remains a challenge because of a low inherent contrast of adjacent edematous 
      brain, the surrounding infiltration zone, and the solid tumor. Therefore, new 
      technologies that may facilitate an intraoperative analysis of the tissue at the 
      resection edge are of great interest to neurosurgeons. MATERIALS AND METHODS: For 
      ex vivo imaging of gliomas in a mouse model and human biopsy specimens of brain 
      tumors and nervous system tissue we have used a time-domain Sirius 713 Tomograph 
      with a central wavelength of 1,310 nm and a coherence length of 15 microm 
      equipped with a mono mode fiber and a modified optical coherence tomography (OCT) 
      adapter containing a lens system for imaging at a working distance of 2.5 cm. A 
      spectral-domain tomograph using 840 nm and 930 nm superluminescence diodes (SLD) 
      with a central wavelength of 900 nm was used as a second imaging modelity. 
      RESULTS: Both time-domain and spectral-domain coherence tomography delineated 
      normal brain, the infiltration zone and solid tumor in murine intracerebral 
      gliomas. Histological evaluation of H&E sections parallel to the optical plain 
      demonstrated that tumor areas of less than a millimeter could be detected and 
      that not only solid tumor, but also brain invaded by a low-density single tumor 
      cells produced an OCT signal different from normal brain. Spectral-domain OCT 
      (SD-OCT) demonstrated a significantly more detailed microstructure of tumor and 
      normal brain up to a tissue depth of 1.5-2.0 mm, whereas the interpretation of 
      time-domain OCT (TD-OCT) was difficult at a tissue depth >1.0 mm. Because of 
      rapid scanning times SD-OCT data could be acquired as 3D data maps, which allowed 
      a multi-planar analysis of the tumor to brain interface. Similar to our findings 
      in experimental gliomas, images of human nervous system tissue acquired using 
      SD-OCT showed a characteristic signal of normal brain tissue and a detailed 
      microstructure of tumor parenchyma. CONCLUSION: Spectral-domain OCT of 
      experimental gliomas and human brain tumor specimens differentiates solid tumor, 
      diffusely invaded brain tissue, and adjacent normal brain based on microstructure 
      and B-scan signal characteristics. In conjunction with the rapid image 
      acquisition rates of SD-OCT, this technology carries the potential of a novel 
      intraoperative imaging tool for the detection of residual tumor and guidance of 
      neurosurgical tumor resections.
CI  - (c) 2006 Wiley-Liss, Inc.
FAU - Bohringer, H J
AU  - Bohringer HJ
AD  - Department of Neurosurgery, University Hospital Schleswig-Holstein, Campus 
      Luebeck, Luebeck, Germany.
FAU - Boller, D
AU  - Boller D
FAU - Leppert, J
AU  - Leppert J
FAU - Knopp, U
AU  - Knopp U
FAU - Lankenau, E
AU  - Lankenau E
FAU - Reusche, E
AU  - Reusche E
FAU - Huttmann, G
AU  - Huttmann G
FAU - Giese, A
AU  - Giese A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lasers Surg Med
JT  - Lasers in surgery and medicine
JID - 8007168
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/*pathology
MH  - Equipment Design
MH  - Glioma/*pathology
MH  - Humans
MH  - Mice
MH  - Time Factors
MH  - *Tomography, Optical Coherence/instrumentation
EDAT- 2006/06/01 09:00
MHDA- 2007/02/21 09:00
CRDT- 2006/06/01 09:00
PHST- 2006/06/01 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/06/01 09:00 [entrez]
AID - 10.1002/lsm.20353 [doi]
PST - ppublish
SO  - Lasers Surg Med. 2006 Jul;38(6):588-97. doi: 10.1002/lsm.20353.