PMID- 16737909
OWN - NLM
STAT- MEDLINE
DCOM- 20060724
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 167
IP  - 2
DP  - 2006 Jun
TI  - Molecular cytogenetic analysis of oral squamous cell carcinomas by comparative 
      genomic hybridization, spectral karyotyping, and fluorescence in situ 
      hybridization.
PG  - 109-16
AB  - We investigated relationships between DNA copy number aberrations and chromosomal 
      structural rearrangements in 11 different cell lines derived from oral squamous 
      cell carcinoma (OSCC) by comparative genomic hybridization (CGH), spectral 
      karyotyping (SKY), and fluorescence in situ hybridization (FISH). CGH frequently 
      showed recurrent chromosomal gains of 5p, 20q12, 8q23 approximately qter, 20p11 
      approximately p12, 7p15, 11p13 approximately p14, and 14q21, as well as losses of 
      4q, 18q, 4p11 approximately p15, 19p13, 8p21 approximately pter, and 16p11 
      approximately p12. SKY identified the following recurrent chromosomal 
      abnormalities: i(5)(p10), i(5)(q10), i(8)(q10), der(X;1)(q10;p10), 
      der(3;5)(p10;p10), and der(3;18)(q10;p10). In addition, breakpoints detected by 
      SKY were clustered in 11q13 and around centromeric regions, including 5p10/q10, 
      3p10/q10, 8p10/q10 14q10, 1p10/1q10, and 16p10/16q10. Cell lines with i(5)(p10) 
      and i(8)(q10) showed gains of the entire chromosome arms of 5p and 8q by CGH. 
      Moreover, breakages near the centromeres of chromosomes 5 and 8 may be associated 
      with 5p gain, 8q gain, and 8p loss in OSCC. FISH with a DNA probe from a BAC 
      clone mapping to 5p15 showed a significant correlation between the average 
      numbers of i(5)(p10) and 5p15 (R(2) = 0.8693, P< 0.01) in these cell lines, 
      indicating that DNA copy number of 5p depends upon isochromosome formation in 
      OSCC.
FAU - Uchida, Kenichiro
AU  - Uchida K
AD  - Department of Oral and Maxillofacial Surgery, 1-1-1 Minami-Kogushi, Ube, 
      Yamaguchi 755-8505, Japan.
FAU - Oga, Atsunori
AU  - Oga A
FAU - Okafuji, Masaki
AU  - Okafuji M
FAU - Mihara, Mariko
AU  - Mihara M
FAU - Kawauchi, Shigeto
AU  - Kawauchi S
FAU - Furuya, Tomoko
AU  - Furuya T
FAU - Chochi, Yasuyo
AU  - Chochi Y
FAU - Ueyama, Yoshiya
AU  - Ueyama Y
FAU - Sasaki, Kohsuke
AU  - Sasaki K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosomes/ultrastructure
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*genetics
MH  - Nucleic Acid Hybridization
MH  - Spectral Karyotyping
EDAT- 2006/06/02 09:00
MHDA- 2006/07/25 09:00
CRDT- 2006/06/02 09:00
PHST- 2005/12/05 00:00 [received]
PHST- 2006/01/18 00:00 [revised]
PHST- 2006/01/25 00:00 [accepted]
PHST- 2006/06/02 09:00 [pubmed]
PHST- 2006/07/25 09:00 [medline]
PHST- 2006/06/02 09:00 [entrez]
AID - S0165-4608(06)00052-5 [pii]
AID - 10.1016/j.cancergencyto.2006.01.007 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Jun;167(2):109-16. doi: 
      10.1016/j.cancergencyto.2006.01.007.