PMID- 16737921
OWN - NLM
STAT- MEDLINE
DCOM- 20060724
LR  - 20071115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 167
IP  - 2
DP  - 2006 Jun
TI  - Loss of TP53 is due to rearrangements involving chromosome region 17p10 
      approximately p12 in chronic lymphocytic leukemia.
PG  - 177-81
AB  - Loss of tumor protein 53 (TP53) has been associated with aggressive disease and 
      poor response to therapy in B-cell chronic lymphocytic leukemia (B-CLL). TP53 is 
      located at chromosome band 17p13 and its absence can be detected by fluorescence 
      in situ hybridization (FISH) in the interphase nuclei of 8-10% patients with 
      B-CLL. To study the cytogenetic mechanism for loss of TP53, metaphase and 
      interphase FISH studies were conducted on 16 B-CLL patients to investigate 17p10 
      to 17p12, a chromosome region known to be rich in low-copy DNA repeats. Loss of 
      TP53 was caused by an isochromosome with breakpoints between 17p10 and 17p11.2 in 
      four patients, an unbalanced translocation involving 17p10 to 17p11.2 in nine 
      patients, and an unbalanced translocation involving 17p11.2 to 17p12 in three 
      patients. These findings indicate that loss of TP53 results from the absence of 
      nearly the entire chromosome 17 p-arm rather than to monosomy 17 or deletions of 
      TP53. Translocations or isochromosome formations at sites of low-copy DNA repeats 
      in 17p10 to 17p12 appear to be the mechanism for the loss of TP53 in B-CLL.
FAU - Fink, Stephanie R
AU  - Fink SR
AD  - Cytogenetics Laboratory, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, 
      USA.
FAU - Smoley, Stephanie A
AU  - Smoley SA
FAU - Stockero, Kimberly J
AU  - Stockero KJ
FAU - Paternoster, Sarah F
AU  - Paternoster SF
FAU - Thorland, Erik C
AU  - Thorland EC
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
FAU - Shanafelt, Tait D
AU  - Shanafelt TD
FAU - Zent, Clive S
AU  - Zent CS
FAU - Call, Timothy G
AU  - Call TG
FAU - Kay, Neil E
AU  - Kay NE
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
GR  - R01 CA95241/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 17/ultrastructure
MH  - Female
MH  - Gene Deletion
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Isochromosomes
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 2006/06/02 09:00
MHDA- 2006/07/25 09:00
CRDT- 2006/06/02 09:00
PHST- 2005/12/20 00:00 [received]
PHST- 2006/01/20 00:00 [accepted]
PHST- 2006/06/02 09:00 [pubmed]
PHST- 2006/07/25 09:00 [medline]
PHST- 2006/06/02 09:00 [entrez]
AID - S0165-4608(06)00050-1 [pii]
AID - 10.1016/j.cancergencyto.2006.01.005 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Jun;167(2):177-81. doi: 
      10.1016/j.cancergencyto.2006.01.005.