PMID- 16737991
OWN - NLM
STAT- MEDLINE
DCOM- 20070104
LR  - 20220330
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 28
IP  - 3
DP  - 2006 Sep
TI  - Characterisation of guinea pig precision-cut lung slices: comparison with human 
      tissues.
PG  - 603-11
AB  - Precision-cut lung slices (PCLS) allow comparison of the airway responses of 
      different species under identical experimental conditions. The aim of this study 
      was to establish and characterise PCLS from guinea pigs (GPs) and to compare them 
      with human PCLS. GP PCLS were prepared according to previously published 
      procedures with the exception that the agarose solution and the initial 
      incubation medium contained isoproterenol to avoid post mortem airway 
      contraction. The median effective concentrations (EC50, expressed as nM) for 
      agonist-induced bronchoconstriction in GP and human PCLS, respectively, were: 
      leukotriene D4 (1.8, 5.0); thromboxane (16, 1.3); serotonin (69, unresponsive); 
      histamine (217, 2,170); and methacholine (231, 234). Allergen-induced 
      bronchoconstriction of passively sensitised PCLS was attenuated by histamine or 
      thromboxane-prostanoid receptor antagonists and was almost completely prevented 
      by their combination with leukotriene receptor antagonists. Airways 
      pre-contracted with methacholine were relaxed by the beta-agonist salbutamol or 
      the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Simultaneous studies 
      of airways and vessels are possible with, for example, EC50 values for 
      endothelin-1 of 37 nM (pulmonary arteries), 10 nM (pulmonary veins) and 9.6 nM 
      (airway). When compared with previous findings in rat and mouse, these data show 
      that guinea pig lungs are a more appropriate model for human airway pharmacology 
      than lungs from rats or mice.
FAU - Ressmeyer, A R
AU  - Ressmeyer AR
AD  - Research Center Borstel, Borstel, Germany.
FAU - Larsson, A K
AU  - Larsson AK
FAU - Vollmer, E
AU  - Vollmer E
FAU - Dahlen, S E
AU  - Dahlen SE
FAU - Uhlig, S
AU  - Uhlig S
FAU - Martin, C
AU  - Martin C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060531
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Bronchoconstrictor Agents)
SB  - IM
MH  - Animals
MH  - Bronchoconstriction
MH  - Bronchoconstrictor Agents/*pharmacology
MH  - Dissection
MH  - Guinea Pigs
MH  - Humans
MH  - In Vitro Techniques
MH  - Lung/*anatomy & histology/*drug effects
MH  - Mice
MH  - Models, Animal
MH  - Rats
EDAT- 2006/06/02 09:00
MHDA- 2007/01/05 09:00
CRDT- 2006/06/02 09:00
PHST- 2006/06/02 09:00 [pubmed]
PHST- 2007/01/05 09:00 [medline]
PHST- 2006/06/02 09:00 [entrez]
AID - 09031936.06.00004206 [pii]
AID - 10.1183/09031936.06.00004206 [doi]
PST - ppublish
SO  - Eur Respir J. 2006 Sep;28(3):603-11. doi: 10.1183/09031936.06.00004206. Epub 2006 
      May 31.