PMID- 16739377
OWN - NLM
STAT- MEDLINE
DCOM- 20110411
LR  - 20161124
VI  - 20
IP  - 6
DP  - 2006 Mar
TI  - [The relationship between hypermethylation of the PTEN promoter and laryngeal 
      squamous cell carcinoma].
PG  - 254-6
AB  - OBJECTIVE: To explore the relationship between hypermethylation of the PTEN gene 
      promoter and laryngeal squamous cell carcinoma (LSCC). METHOD: Promoter 
      hypermethylation and mRNA expression were detected by methylation specific PCR 
      and RT-PCR in LSCC and normal laryngeal tissue. RESULT: Among the 40 patients 
      with LSCC, hypermethylation of PTEN gene promoter was detected in 16 cases 
      (40.0%). Among the nine normal laryngeal tissues, hypermethylation of PTEN gene 
      promoter was detected in no case. The high and moderate expression rates of PTEN 
      gene mRNA in nine normal laryngeal tissues were 77.8% (7/9) and 22.2% (2/9), 
      respectively; The high, moderate, low and negative expression rates of PTEN mRNA 
      in 15 laryngeal cancers with lymph node metastasis were 26.7% (4/15), 33.3% 
      (5/15), 26.7% (4/15) and 13.3% (2/15), respectively. The PTEN mRNA expression in 
      normal laryngeal tissues was higher than that in the laryngeal cancers with lymph 
      node metastasis (P < 0.05). The high, moderate and low expression rates of PTEN 
      mRNA in 10 highly differentiated laryngeal cancers were 60.0% (6/10), 30.0% 
      (3/10) and 10.0% (1/10), respectively; The high, moderate, low and negative 
      expression rates of PTEN mRNA in 12 low differentiated laryngeal cancers were 
      16.7% (2/12), 25.0% (3/12), 41.7% (5/12) and 16.7% (2/12), respectively. The 
      differences of PTEN mRNA expression between high and low differentiation 
      laryngeal cancers were statistically significant (P < 0.05). The hypermethylation 
      rate of PTEN gene promoter in the low mRNA expression laryngeal cancers was 70.0% 
      (7/10), the hypermethylation rate of PTEN gene promoter in the high mRNA 
      expression laryngeal cancers was 23.5% (4/17). The hypermethylation rate of PTEN 
      gene promoter in the low mRNA expression laryngeal cancers was higher than that 
      in the high mRNA expression laryngeal cancers (P < 0.05). CONCLUSION: 
      Hypermethylation of PTEN gene promoter is associated with loss of its 
      transcription in laryngeal squamous cell carcinoma, the decreasing of PTEN mRNA 
      expression may be related to lymph node metastasis and low differentiation of 
      laryngeal squamous cell carcinoma.
FAU - Bai, Weiliang
AU  - Bai W
AD  - Department of Otolaryngology, the Second Affiliated Hospital of China Medical 
      University, Shenyang, 110004, China. bweiliang@yahoo.com.cn
FAU - Li, Wei
AU  - Li W
FAU - Chen, Xiaoqiu
AU  - Chen X
FAU - Wang, Tie
AU  - Wang T
LA  - chi
PT  - Journal Article
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Ke Za Zhi
JT  - Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
JID - 9426080
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism
MH  - Case-Control Studies
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - PTEN Phosphohydrolase/genetics/*metabolism
MH  - *Promoter Regions, Genetic
EDAT- 2006/06/03 09:00
MHDA- 2011/04/13 06:00
CRDT- 2006/06/03 09:00
PHST- 2006/06/03 09:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
PHST- 2006/06/03 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Mar;20(6):254-6.