PMID- 16739577
OWN - NLM
STAT- MEDLINE
DCOM- 20060623
LR  - 20220331
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 323
DP  - 2006
TI  - Cytogenetic analyses of Arabidopsis.
PG  - 173-86
AB  - For a long time, Arabidopsis thaliana was not a favorite of cytogeneticists 
      because of its small chromosomes. Nevertheless, most cytogenetic approaches have 
      become applicable to this model plant during the past decade. Recently, the very 
      small genome, the low content, and strong clustering of repetitive sequences even 
      turned out to be advantageous for the first establishment of chromosome painting 
      in a euploid plant. Chromosome painting together with the high-resolution 
      fluorescence in situ hybridization (FISH) mapping on Arabidopsis pachytene 
      chromosomes has opened new perspectives for plant cytogenetics. In this chapter, 
      we describe standard cytogenetic methods including preparation of chromosomes and 
      chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, 
      painting of Arabidopsis chromosomes, and comparative chromosome painting in other 
      Brassicaceae species.
FAU - Lysak, Martin
AU  - Lysak M
AD  - Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, UK.
FAU - Fransz, Paul
AU  - Fransz P
FAU - Schubert, Ingo
AU  - Schubert I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Arabidopsis/*metabolism
MH  - Brassicaceae/genetics
MH  - Chromosome Mapping
MH  - Chromosome Painting
MH  - Chromosomes, Plant
MH  - Cytogenetic Analysis
MH  - Cytogenetics/*methods
MH  - Genes, Plant
MH  - *Genetic Techniques
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - *Models, Genetic
EDAT- 2006/06/03 09:00
MHDA- 2006/06/24 09:00
CRDT- 2006/06/03 09:00
PHST- 2006/06/03 09:00 [pubmed]
PHST- 2006/06/24 09:00 [medline]
PHST- 2006/06/03 09:00 [entrez]
AID - 10.1385/1-59745-003-0:173 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;323:173-86. doi: 10.1385/1-59745-003-0:173.