PMID- 16739953
OWN - NLM
STAT- MEDLINE
DCOM- 20060828
LR  - 20131121
IS  - 8756-7938 (Print)
IS  - 1520-6033 (Linking)
VI  - 22
IP  - 3
DP  - 2006 May-Jun
TI  - Differential gene expression profiles and real-time measurements of growth 
      parameters in Saccharomyces cerevisiae grown in microliter-scale bioreactors 
      equipped with internal stirring.
PG  - 710-7
AB  - Combining real-time growth kinetics measurements with global gene expression 
      analysis of microbial cultures is of significant value for high-throughput 
      biological research. We have performed differential gene expression analysis in 
      the eukaryotic model Saccharomyces cerevisiae grown in galactose and glucose 
      media in 150 muL bioreactors equipped with sensors for in situ and real-time 
      measurements of optical density (OD), pH, and dissolved oxygen (DO). The 
      microbioreactors were fabricated from poly(dimethylsiloxane) (PDMS) and 
      poly(methyl methacrylate) (PMMA) and equipped with internal magnetic ministirrers 
      and evaporation compensation by water replacement. In galactose-grown cells, the 
      core genes of the GAL operon GAL2, GAL1, GAL7, and GAL10 were upregulated at 
      least 100-fold relative to glucose-grown cells. These differential gene 
      expression levels were similar to those observed in large-scale culture vessels. 
      The increasing rate at which complete genomic sequences of microorganisms are 
      becoming available offers an unprecedented opportunity for comparative 
      investigations of these organisms. Our results from S. cerevisiae cultures grown 
      in instrumented microbioreactors show that it is possible to integrate 
      high-throughput studies of growth physiology with global gene expression analysis 
      of microorganisms.
FAU - Boccazzi, Paolo
AU  - Boccazzi P
AD  - Department of Biology and Health Sciences and Technology, Massachusetts Institute 
      of Technology, Cambridge, Massachusetts 02139, USA.
FAU - Zhang, Zhiyu
AU  - Zhang Z
FAU - Kurosawa, Kazuhiko
AU  - Kurosawa K
FAU - Szita, Nicolas
AU  - Szita N
FAU - Bhattacharya, Sanchita
AU  - Bhattacharya S
FAU - Jensen, Klavs F
AU  - Jensen KF
FAU - Sinskey, Anthony J
AU  - Sinskey AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 63231-63-0 (RNA)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - *Bioreactors
MH  - Cell Culture Techniques/methods
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Equipment Design
MH  - Equipment Failure Analysis/methods
MH  - Galactose/chemistry/pharmacology
MH  - *Gene Expression Profiling
MH  - Glucose/chemistry/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Microarray Analysis/instrumentation/*methods
MH  - Models, Biological
MH  - Oxygen/chemistry
MH  - RNA/isolation & purification
MH  - Saccharomyces cerevisiae/*genetics/*growth & development/metabolism
MH  - Time Factors
EDAT- 2006/06/03 09:00
MHDA- 2006/08/29 09:00
CRDT- 2006/06/03 09:00
PHST- 2006/06/03 09:00 [pubmed]
PHST- 2006/08/29 09:00 [medline]
PHST- 2006/06/03 09:00 [entrez]
AID - 10.1021/bp0504288 [doi]
PST - ppublish
SO  - Biotechnol Prog. 2006 May-Jun;22(3):710-7. doi: 10.1021/bp0504288.