PMID- 16740358
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20240109
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 247
IP  - 1
DP  - 2007 Mar 8
TI  - The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and 
      induces apoptosis.
PG  - 150-8
AB  - C-Phycocyanin (C-PC) from blue-green algae has been reported to have various 
      pharmacological characteristics, including anti-inflammatory and anti-tumor 
      activities. In this study, we expressed the beta-subunit of C-PC (ref to as 
      C-PC/beta) in Escherichia coli. We found that the recombinant C-PC/beta has 
      anti-cancer properties. Under the treatment of 5 microM of the recombinant 
      C-PC/beta, four different cancer cell lines accrued high proliferation inhibition 
      and apoptotic induction. Substantially, a lower response occurred in non-cancer 
      cells. We investigated the mechanism by which C-PC/beta inhibits cancer cell 
      proliferation and induces apoptosis. We found that the C-PC/beta interacts with 
      membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH). Under the treatment of the C-PC/beta, depolymerization of microtubules 
      and actin-filaments were observed. The cells underwent apoptosis with an increase 
      in caspase-3, and caspase-8 activities. The cell cycle was arrested at the G0/G1 
      phase under the treatment of C-PC/beta. In addition, the nuclear level of GAPDH 
      decreased significantly. Decrease in the nuclear level of GAPDH prevents the cell 
      cycle from entering into the S phase. Inhibition of cancer cell proliferation and 
      induction of apoptosis may potentate the C-PC/beta as a promising cancer 
      prevention or therapy agent.
FAU - Wang, Haizhen
AU  - Wang H
AD  - Department of Biology, Georgia Cancer Center, Georgia State University, 
      University Plaza, Atlanta, GA 30303, USA.
FAU - Liu, Yongding
AU  - Liu Y
FAU - Gao, Xueliang
AU  - Gao X
FAU - Carter, Christie L
AU  - Carter CL
FAU - Liu, Zhi-Ren
AU  - Liu ZR
LA  - eng
GR  - GM063874/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060605
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Protein Subunits)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tubulin)
RN  - 11016-15-2 (Phycocyanin)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Antineoplastic Agents
MH  - Apoptosis/drug effects
MH  - Caspases/metabolism
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - Microtubules/metabolism
MH  - Phycocyanin/chemistry/genetics/pharmacology/*therapeutic use
MH  - Protein Subunits/genetics/therapeutic use
MH  - Recombinant Proteins/therapeutic use
MH  - Tubulin
EDAT- 2006/06/03 09:00
MHDA- 2007/03/21 09:00
CRDT- 2006/06/03 09:00
PHST- 2006/01/16 00:00 [received]
PHST- 2006/03/31 00:00 [revised]
PHST- 2006/04/06 00:00 [accepted]
PHST- 2006/06/03 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2006/06/03 09:00 [entrez]
AID - S0304-3835(06)00215-1 [pii]
AID - 10.1016/j.canlet.2006.04.002 [doi]
PST - ppublish
SO  - Cancer Lett. 2007 Mar 8;247(1):150-8. doi: 10.1016/j.canlet.2006.04.002. Epub 
      2006 Jun 5.