PMID- 16751372
OWN - NLM
STAT- MEDLINE
DCOM- 20060713
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 176
IP  - 12
DP  - 2006 Jun 15
TI  - IL-2 is required for the activation of memory CD8+ T cells via antigen 
      cross-presentation.
PG  - 7288-300
AB  - Dendritic cells (DCs) are capable of capturing exogenous Ag for the generation of 
      MHC class I/peptide complexes. For efficient activation of memory CD8(+) T cells 
      to occur via a cross-presentation pathway, DCs must receive helper signals from 
      CD4(+) T cells. Using an in vitro system that reflects physiologic recall memory 
      responses, we have evaluated signals that influence helper-dependent 
      cross-priming, while focusing on the source and cellular target of such effector 
      molecules. Concerning the interaction between CD4(+) T cells and DCs, we tested 
      the hypothesis that CD40 engagement on DCs is critical for IL-12p70 (IL-12) 
      production and subsequent stimulation of IFN-gamma release by CD8(+) T cells. 
      Although CD40 engagement on DCs, or addition of exogenous IL-12 are both 
      sufficient to overcome the lack of help, neither is essential. We next evaluated 
      cytokines and chemokines produced during CD4(+) T cell/DC cross talk and observed 
      high levels of IL-2 produced within the first 18-24 h of Ag-specific T cell 
      engagement. Functional studies using blocking Abs to CD25 completely abrogated 
      IFN-gamma production by the CD8(+) T cells. Although required, addition of 
      exogenous IL-2 did not itself confer signals sufficient to overcome the lack of 
      CD4(+) T cell help. Thus, these data support a combined role for Ag-specific, 
      cognate interactions at the CD4(+) T cell/DC as well as the DC/CD8(+) T cell 
      interface, with the helper effect mediated by soluble noncognate signals.
FAU - Blachere, Nathalie E
AU  - Blachere NE
AD  - The Rockefeller University, New York, NY 10021, USA.
FAU - Morris, Heather K
AU  - Morris HK
FAU - Braun, Deborah
AU  - Braun D
FAU - Saklani, Helene
AU  - Saklani H
FAU - Di Santo, James P
AU  - Di Santo JP
FAU - Darnell, Robert B
AU  - Darnell RB
FAU - Albert, Matthew L
AU  - Albert ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Viral)
RN  - 0 (CD40 Antigens)
RN  - 0 (Interleukin-2)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antigens, Viral/immunology/*metabolism
MH  - CD40 Antigens/immunology/metabolism/physiology
MH  - CD8-Positive T-Lymphocytes/*immunology/*metabolism/virology
MH  - Cell Differentiation/genetics/immunology
MH  - Cells, Cultured
MH  - *Cross-Priming/genetics
MH  - Dendritic Cells/cytology/immunology/metabolism
MH  - *Immunologic Memory/genetics
MH  - Influenza A virus/*immunology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-2/deficiency/genetics/*physiology
MH  - *Lymphocyte Activation/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Receptors, Interleukin/physiology
MH  - Receptors, Interleukin-12
MH  - Signal Transduction/genetics/immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology/virology
EDAT- 2006/06/06 09:00
MHDA- 2006/07/14 09:00
CRDT- 2006/06/06 09:00
PHST- 2006/06/06 09:00 [pubmed]
PHST- 2006/07/14 09:00 [medline]
PHST- 2006/06/06 09:00 [entrez]
AID - 176/12/7288 [pii]
AID - 10.4049/jimmunol.176.12.7288 [doi]
PST - ppublish
SO  - J Immunol. 2006 Jun 15;176(12):7288-300. doi: 10.4049/jimmunol.176.12.7288.