PMID- 16751493
OWN - NLM
STAT- MEDLINE
DCOM- 20060830
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 6
DP  - 2006 Jun
TI  - Microbial diversity in Maras salterns, a hypersaline environment in the Peruvian 
      Andes.
PG  - 3887-95
AB  - Maras salterns are located 3,380 m above sea level in the Peruvian Andes. These 
      salterns consist of more than 3,000 little ponds which are not interconnected and 
      act as crystallizers where salt precipitates. These ponds are fed by hypersaline 
      spring water rich in sodium and chloride. The microbiota inhabiting these 
      salterns was examined by fluorescence in situ hybridization (FISH), 16S rRNA gene 
      clone library analysis, and cultivation techniques. The total counts per 
      milliliter in the ponds were around 2 x 10(6) to 3 x 10(6) cells/ml, while the 
      spring water contained less than 100 cells/ml and did not yield any detectable 
      FISH signal. The microbiota inhabiting the ponds was dominated (80 to 86% of the 
      total counts) by Archaea, while Bacteria accounted for 10 to 13% of the 
      4',6'-diamidino-2-phenylindole (DAPI) counts. A total of 239 16S rRNA gene clones 
      were analyzed (132 Archaea clones and 107 Bacteria clones). According to the 
      clone libraries, the archaeal assemblage was dominated by microorganisms related 
      to the cosmopolitan square archaeon "Haloquadra walsbyi," although a substantial 
      number of the sequences in the libraries (31% of the 16S rRNA gene archaeal 
      clones) were related to Halobacterium sp., which is not normally found in clone 
      libraries from solar salterns. All the bacterial clones were closely related to 
      each other and to the gamma-proteobacterium "Pseudomonas halophila" DSM 3050. 
      FISH analysis with a probe specific for this bacterial assemblage revealed that 
      it accounted for 69 to 76% of the total bacterial counts detected with a 
      Bacteria-specific probe. When pond water was used to inoculate solid media 
      containing 25% total salts, both extremely halophilic Archaea and Bacteria were 
      isolated. Archaeal isolates were not related to the isolates in clone libraries, 
      although several bacterial isolates were very closely related to the "P. 
      halophila" cluster found in the libraries. As observed for other hypersaline 
      environments, extremely halophilic bacteria that had ecological relevance seemed 
      to be easier to culture than their archaeal counterparts.
FAU - Maturrano, Lenin
AU  - Maturrano L
AD  - Division de Microbiologia, Departamento de Fisiologia, Genetica y Microbiologia, 
      Universidad de Alicante, Apto. 99, San Vicente del Raspeig, 03080 Alicante, 
      Spain.
FAU - Santos, Fernando
AU  - Santos F
FAU - Rossello-Mora, Ramon
AU  - Rossello-Mora R
FAU - Anton, Josefa
AU  - Anton J
LA  - eng
SI  - GENBANK/AY987818
SI  - GENBANK/AY987819
SI  - GENBANK/AY987820
SI  - GENBANK/AY987821
SI  - GENBANK/AY987822
SI  - GENBANK/AY987823
SI  - GENBANK/AY987824
SI  - GENBANK/AY987825
SI  - GENBANK/AY987826
SI  - GENBANK/AY987827
SI  - GENBANK/AY987828
SI  - GENBANK/AY987829
SI  - GENBANK/AY987830
SI  - GENBANK/AY987831
SI  - GENBANK/AY987832
SI  - GENBANK/AY987833
SI  - GENBANK/AY987834
SI  - GENBANK/AY987835
SI  - GENBANK/AY987836
SI  - GENBANK/AY987837
SI  - GENBANK/AY987838
SI  - GENBANK/AY987839
SI  - GENBANK/AY987840
SI  - GENBANK/AY987841
SI  - GENBANK/AY987842
SI  - GENBANK/AY987843
SI  - GENBANK/AY987844
SI  - GENBANK/AY987845
SI  - GENBANK/AY987846
SI  - GENBANK/AY987847
SI  - GENBANK/AY987848
SI  - GENBANK/AY987849
SI  - GENBANK/AY987850
SI  - GENBANK/AY987851
SI  - GENBANK/DQ019934
SI  - GENBANK/DQ019935
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Altitude
MH  - DNA, Archaeal/genetics/isolation & purification
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Fresh Water/*microbiology
MH  - *Genetic Variation
MH  - Halobacterium/classification/genetics/isolation & purification
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Peru
MH  - Phylogeny
MH  - Pseudomonas/classification/genetics/isolation & purification
MH  - Rhodospirillum/classification/genetics
MH  - Water Microbiology
PMC - PMC1489619
EDAT- 2006/06/06 09:00
MHDA- 2006/08/31 09:00
PMCR- 2006/10/01
CRDT- 2006/06/06 09:00
PHST- 2006/06/06 09:00 [pubmed]
PHST- 2006/08/31 09:00 [medline]
PHST- 2006/06/06 09:00 [entrez]
PHST- 2006/10/01 00:00 [pmc-release]
AID - 72/6/3887 [pii]
AID - 2214-05 [pii]
AID - 10.1128/AEM.02214-05 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Jun;72(6):3887-95. doi: 10.1128/AEM.02214-05.