PMID- 16752002
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20221207
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 8
IP  - 5
DP  - 2006 Sep
TI  - Porcine vesical acellular matrix graft of tunica albuginea for penile 
      reconstruction.
PG  - 543-8
AB  - AIM: To characterize the feasibility of the surgical replacement of the penile 
      tunica albuginea (TA) and to evaluate the value of a porcine bladder acellular 
      matrix (BAM) graft. METHODS: Acellular matrices were constructed from pigs' 
      bladders by cell lysis, and then examined by scanning electron microscopy (SEM). 
      Expression levels of the mRNA of the vascular endothelial growth factor (VEGF) 
      receptor, fibroblast growth factor (FGF)-1 receptor, neuregulin, and 
      brain-derived neurotrophic factor (BDNF) in the acellular matrix and submucosa of 
      the pigs'bladders were determined through the reverse transcription-polymerase 
      chain reaction (PCR). A 5 mm X 5 mm square was excised from the penile TA of nine 
      rabbits. The defective TA was then covered in porcine BAM. Equal numbers of 
      animals were sacrificed and histochemically examined at 2, 4 and 6 months after 
      implantation. RESULTS: SEM of the BAM showed collagen fibers with many pores. 
      VEGF receptor, FGF-1 receptor and neuregulin mRNA were expressed in the porcine 
      BAM; BDNF mRNA was not detected. Two months after implantation, the graft sites 
      exhibited excellent healing without contracture, and the fusion between the graft 
      and the neighboring normal TA appeared to be well established. There were no 
      significant histological differences between the implanted tunica and the normal 
      control tunica at 6 months after implantation. CONCLUSION: The porcine BAM graft 
      resulted in a structure which was sufficiently like that of the normal TA. This 
      implantation might be considered applicable to the reconstruction of the TA in 
      conditions such as trauma or Peyronie's disease.
FAU - Joo, Kwan-Joong
AU  - Joo KJ
AD  - Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School 
      of Medicine, 108 Pyung-Dong, Jongro-Ku, Seoul 110-746, Korea.
FAU - Kim, Byung-Soo
AU  - Kim BS
FAU - Han, Jeong-Ho
AU  - Han JH
FAU - Kim, Chang-Ju
AU  - Kim CJ
FAU - Kwon, Chil-Hun
AU  - Kwon CH
FAU - Park, Heung-Jae
AU  - Park HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060605
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuregulins)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
RN  - EC 5.2.1.- (Cyclophilins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Cyclophilins/genetics
MH  - Disease Models, Animal
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Neuregulins/genetics
MH  - Penis/*surgery
MH  - Polymerase Chain Reaction
MH  - Receptors, Fibroblast Growth Factor/genetics
MH  - Receptors, Vascular Endothelial Growth Factor/genetics
MH  - Plastic Surgery Procedures
MH  - Surgery, Plastic
MH  - Swine
MH  - Urinary Bladder/physiology/*surgery/ultrastructure
EDAT- 2006/06/06 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/06/06 09:00
PHST- 2006/06/06 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/06/06 09:00 [entrez]
AID - 10.1111/j.1745-7262.2006.00192.x [doi]
PST - ppublish
SO  - Asian J Androl. 2006 Sep;8(5):543-8. doi: 10.1111/j.1745-7262.2006.00192.x. Epub 
      2006 Jun 5.