PMID- 16753602
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20131121
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 126
IP  - 1
DP  - 2006 Jul
TI  - Molecular characterization of chronic lymphocytic leukemia with two distinct cell 
      populations: Evidence for separate clonal origins.
PG  - 23-8
AB  - We report a case of an elderly woman with persistent lymphocytosis in whom flow 
      cytometric immunophenotyping revealed 2 distinct clonal B-cell populations with 
      different light chain restrictions. One clone was CD19+ (dim), CD5+ (bright), 
      CD23+ (moderate), and kappa+; the other clone was CD19+ (moderate), CD5+ 
      (bright), CD23+ (dim), and lambda+. We separated the 2 clones by flow cytometry 
      from peripheral blood lymphocytes for further molecular and cytogenetic analysis. 
      Polymerase chain reaction (PCR) analysis of the immunoglobulin heavy chain (IgH) 
      genes revealed that the clones had bands of slightly different size. The PCR 
      products of the framework 3 (FR3) region were cloned, and sequence analysis 
      confirmed that each population had distinct, clone-specific IgH gene 
      rearrangements. Fluorescence in situ hybridization (FISH) analysis revealed 
      trisomy 12 in the lambda-restricted B-cell clone, whereas the k-restricted 
      population had normal FISH patterns. Our results demonstrated that the 
      immunophenotypically different cell populations originated from 2 separate 
      clones.
FAU - Chang, Hong
AU  - Chang H
AD  - Department of Laboratory Hematology, University Health Network and University of 
      Toronto, Toronto, Canada.
FAU - Cerny, Jan
AU  - Cerny J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, CD)
RN  - 0 (Antineoplastic Agents, Alkylating)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 18D0SL7309 (Chlorambucil)
SB  - IM
MH  - Aged, 80 and over
MH  - Antigens, CD/metabolism
MH  - Antineoplastic Agents, Alkylating/therapeutic use
MH  - Cell Differentiation
MH  - Cell Lineage/*genetics/immunology
MH  - Chlorambucil/therapeutic use
MH  - Clone Cells/immunology/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Rearrangement/genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/drug 
      therapy/*genetics/immunology/*pathology
EDAT- 2006/06/07 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/06/07 09:00
PHST- 2006/06/07 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/06/07 09:00 [entrez]
AID - 0YYF17GFKFJFNP5G [pii]
AID - 10.1309/0YYF-17GF-KFJF-NP5G [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2006 Jul;126(1):23-8. doi: 10.1309/0YYF-17GF-KFJF-NP5G.