PMID- 16753805
OWN - NLM
STAT- MEDLINE
DCOM- 20060914
LR  - 20081121
IS  - 0300-8207 (Print)
IS  - 0300-8207 (Linking)
VI  - 47
IP  - 3
DP  - 2006
TI  - Bone morphogenetic protein-2 counterregulates interleukin-18 mRNA and protein in 
      MC3T3-E1 mouse osteoblastic cells.
PG  - 124-32
AB  - Fibroblast growth factors-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) are 
      two of the main factors that regulate differentiation of osteoblasts. 
      Interleukin-18 (IL-18), originally cloned as an interferon gamma-inducing factor, 
      has been reported to inhibit maturation of osteoclasts by upregulation of 
      osteoprotegerin secreted from osteoblasts. Little is known about the functional 
      relationship between IL-18 and the two growth factors in osteoblast 
      differentiation. To better understand this relationship, we analyzed the effect 
      of BMP-2 and FGF-2 on the mRNA expression levels of IL-18, as well as IL-1alpha 
      and IL-6, in MC3T3-E1 mouse osteoblastic cells. Following this, the effects of 
      BMP-2 on the expression of IL-18 protein and caspase-1 protein were analyzed by 
      immunofluorescence staining. Real-time PCR and immunofluorescence staining 
      analysis showed that FGF-2 had no effect on the expression of IL-18 mRNA and 
      protein, but while BMP-2 reduced IL-18 mRNA levels, increased immunostaining of 
      both IL-18 protein and caspase-1 protein was detected in BMP-2-treated MC3T3-E1 
      cells. Although the significance and mechanisms of this counterregulation of 
      IL-18 mRNA and protein were not determined in this study, the increase of IL-18 
      protein suggested that BMP-2 may induce an active form of IL-18.
FAU - Hori, Masateru
AU  - Hori M
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Hyogo, Japan.
FAU - Sawai, Hideaki
AU  - Sawai H
FAU - Tsuji, Yoshiyuki
AU  - Tsuji Y
FAU - Okamura, Haruki
AU  - Okamura H
FAU - Koyama, Koji
AU  - Koyama K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Caspase 1/metabolism
MH  - Cell Line
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Interleukin-1/genetics/metabolism
MH  - Interleukin-18/*genetics/metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - Mice
MH  - Osteoblasts/*drug effects/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Time Factors
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2006/06/07 09:00
MHDA- 2006/09/15 09:00
CRDT- 2006/06/07 09:00
PHST- 2006/06/07 09:00 [pubmed]
PHST- 2006/09/15 09:00 [medline]
PHST- 2006/06/07 09:00 [entrez]
AID - VN7316QR30358282 [pii]
AID - 10.1080/03008200600685350 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2006;47(3):124-32. doi: 10.1080/03008200600685350.