PMID- 16753837
OWN - NLM
STAT- MEDLINE
DCOM- 20060801
LR  - 20131121
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 40
IP  - 6
DP  - 2006 Jun
TI  - Helicobacter hepaticus Dps protein plays an important role in protecting DNA from 
      oxidative damage.
PG  - 597-605
AB  - The ferritin-like DNA-binding protein from starved cells (Dps) family proteins 
      are present in a number of pathogenic bacteria. Dps in the enterohepatic 
      pathogen, Helicobacter hepaticus is characterized and a H. hepaticus dps mutant 
      was generated by insertional mutagenesis. While the wild type H. hepaticus cells 
      were able to survive in an atmosphere containing up to 6.0% O2, the dps mutant 
      failed to grow in 3.0% O2, and it was also more sensitive to oxidative reagents 
      like H2O2, cumene hydroperoxide and t-butyl hydroperoxide. Upon air exposure, the 
      dps- cells had more damaged DNA than the wild type; they became coccoid or lysed 
      and they contained approximately 6-fold higher amount of 8-oxoguanine (8-oxoG) 
      DNA lesions than wild type cells. Purified H. hepaticus Dps was shown to be able 
      to bind both iron and DNA. The iron-loaded form of Dps protein had much greater 
      DNA binding ability than the native Dps or the iron-free Dps.
FAU - Hong, Yang
AU  - Hong Y
AD  - Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
FAU - Wang, Ge
AU  - Wang G
FAU - Maier, Robert J
AU  - Maier RJ
LA  - eng
GR  - DK60061/DK/NIDDK NIH HHS/United States
GR  - DK62852/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DPS protein, Bacteria)
RN  - 5614-64-2 (8-hydroxyguanine)
RN  - 5Z93L87A1R (Guanine)
RN  - 9007-49-2 (DNA)
RN  - E1UOL152H7 (Iron)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bacterial Proteins/genetics/isolation & purification/*metabolism
MH  - Cell Shape
MH  - DNA/*metabolism
MH  - *DNA Damage
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Guanine/analogs & derivatives/metabolism
MH  - Helicobacter hepaticus/cytology/genetics/*metabolism
MH  - Iron/metabolism
MH  - Mutation/genetics
MH  - Oxidation-Reduction
MH  - Oxygen/metabolism
MH  - Protein Binding
EDAT- 2006/06/07 09:00
MHDA- 2006/08/02 09:00
CRDT- 2006/06/07 09:00
PHST- 2006/06/07 09:00 [pubmed]
PHST- 2006/08/02 09:00 [medline]
PHST- 2006/06/07 09:00 [entrez]
AID - G7L424W113080253 [pii]
AID - 10.1080/10715760600618882 [doi]
PST - ppublish
SO  - Free Radic Res. 2006 Jun;40(6):597-605. doi: 10.1080/10715760600618882.