PMID- 16753847
OWN - NLM
STAT- MEDLINE
DCOM- 20060818
LR  - 20191210
IS  - 1024-5332 (Print)
IS  - 1024-5332 (Linking)
VI  - 11
IP  - 2
DP  - 2006 Apr
TI  - A study for evaluation of different diagnostic approaches in acute leukemia in 
      Egypt.
PG  - 87-95
AB  - Cytomorphology, cytochemistry, immunophenotyping, in addition to cytogenetic and 
      molecular analyses have specific roles in the diagnosis and management of acute 
      leukemias. This work was designed as a comparative study of different available 
      methods for diagnosis of acute leukemia. The study comprised 47 cases with acute 
      leukemia (21 cases with ALL and 26 cases with AML). Peripheral blood and bone 
      marrow samples were subjected to through morphological examination of 
      Leishman-stained smears, cytochemical analysis, immunophenotyping, conventional 
      cytogenetic banding analysis, fluorescence in situ hybridization (FISH) for 
      selected cases, and RT-PCR for detection of BCR-ABL rearrangement. The results of 
      the study revealed that careful examination of Romanowsky-stained peripheral 
      blood and BM films is fundamental in the diagnosis of acute leukemias, and when 
      considered together with clinical and hematological features, indicates which of 
      the more specialized techniques are most likely to be useful. The major role of 
      cytochemistry was in the diagnosis of AML, while the major role of 
      immunophenotyping was in the diagnosis of acute leukemia, which is not obviously 
      myeloid. Apart from identification of chromosomal abnormalities unique to 
      specific subtypes of leukemia, cytogenetic analysis had a salient impact on 
      anticipating the prognosis and treatment outcome in acute leukemias. We could 
      conclude that the techniques used in this study are considered complementary 
      rather than alternatives and that stepwise employment of strategies is more cost 
      effective than doing all the tests simultaneously.
FAU - Mahmoud, Lotfy A
AU  - Mahmoud LA
AD  - Mansoura University, Hematology Unit of Clinical Pathology Department, Mansoura, 
      Egypt.
FAU - Shamaa, Sameh S A
AU  - Shamaa SS
FAU - Salem, Mohammad A
AU  - Salem MA
FAU - Aladle, Doaa A
AU  - Aladle DA
FAU - Goda, Enas F A
AU  - Goda EF
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Analysis of Variance
MH  - Female
MH  - Genetic Markers
MH  - Histocytochemistry/methods
MH  - Humans
MH  - Immunophenotyping/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*diagnosis/genetics
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis/genetics
MH  - Prognosis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Statistics, Nonparametric
EDAT- 2006/06/07 09:00
MHDA- 2006/08/19 09:00
CRDT- 2006/06/07 09:00
PHST- 2006/06/07 09:00 [pubmed]
PHST- 2006/08/19 09:00 [medline]
PHST- 2006/06/07 09:00 [entrez]
AID - U032R64756076564 [pii]
AID - 10.1080/10245330600574300 [doi]
PST - ppublish
SO  - Hematology. 2006 Apr;11(2):87-95. doi: 10.1080/10245330600574300.