PMID- 16753896
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20061115
IS  - 0020-7454 (Print)
IS  - 0020-7454 (Linking)
VI  - 116
IP  - 6
DP  - 2006 Jun
TI  - Neostriatal cytoskeleton changes following perinatal asphyxia: effect of 
      hypothermia treatment.
PG  - 697-714
AB  - Long-term changes of different types of neurofilaments (NF) and glial fibrillar 
      acid protein (GFAP) were studied in neostriatal rat subjected to perinatal 
      asphyxia (PA) under normothermic and hypothermic (15 degrees C) conditions, using 
      immunohistochemistry for light and electron microscopy. Neostriatal neurons of 
      6-month-old rats that were subjected to 19 and 20 min of PA, showed an increase 
      of NF 200 kDa immunostaining mainly in the axon fascicles in comparison with the 
      control and hypothermia groups. In contrast, no alterations were seen with NF68 
      and NF160 neurofilament antibodies. Furthermore, the same PA groups showed 
      astroglial cells with enhanced GFAP immunoreactivity, evidencing a typical 
      astroglial reaction with a clear hypertrophy of these cells. A quantitative image 
      analysis confirmed these observations. Hypothermic treated animals did show 
      neither astroglial nor neuronal cytoskeletal changes in comparison to the control 
      group. These findings showed that PA produces chronic cytoskeletal alterations in 
      the neostriatum cells that can be prevented by hypothermia.
FAU - Cebral, Elisa
AU  - Cebral E
AD  - Instituto de Biologia Celular y Neurociencias Prof. E. De Robertis, Facultad de 
      Medicina Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Capani, Francisco
AU  - Capani F
FAU - Selvin-Testa, Asia
AU  - Selvin-Testa A
FAU - Funes, Manuel Rey
AU  - Funes MR
FAU - Coirini, Hector
AU  - Coirini H
FAU - Loidl, C Fabian
AU  - Loidl CF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Neurosci
JT  - The International journal of neuroscience
JID - 0270707
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Asphyxia/*metabolism/therapy
MH  - Cytoskeleton/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - *Hypothermia
MH  - Image Processing, Computer-Assisted/methods
MH  - Immunohistochemistry/methods
MH  - Male
MH  - Microscopy, Immunoelectron/methods
MH  - Neostriatum/*metabolism/ultrastructure
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 2006/06/07 09:00
MHDA- 2006/07/27 09:00
CRDT- 2006/06/07 09:00
PHST- 2006/06/07 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/06/07 09:00 [entrez]
AID - R56VW6440L602154 [pii]
AID - 10.1080/00207450600674970 [doi]
PST - ppublish
SO  - Int J Neurosci. 2006 Jun;116(6):697-714. doi: 10.1080/00207450600674970.