PMID- 1675560
OWN - NLM
STAT- MEDLINE
DCOM- 19910712
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 118
IP  - 1-2
DP  - 1991
TI  - Characterization of two human lymphoid cell lines producing human T-lymphotropic 
      virus type I (HTLV-I) isolated from patients with HTLV-I-associated myelopathy or 
      encephalopathy.
PG  - 101-12
AB  - Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented 
      cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type 
      I (HTLV-I) antibody-positive male patients with encephalopathy and 
      HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear 
      cells from a healthy seronegative female. These cell lines, possessing a normal 
      female karyotype, revealed similar characteristics as follows; they expressed 
      HTLV-I-related antigens, they produced C-type retrovirus particles, HTLV-I 
      provirus genomes were integrated into their DNAs, and they had CD4+ activated 
      T-cell markers. In addition, immunocytochemical and immunoelectron microscopic 
      studies showed peculiar immunoreactivity of these cell lines with anti-alpha/beta 
      T-cell antigen receptor (TCR) antibodies; beta Fl, defining beta chain epitope, 
      was only positive in the perinuclear spaces and rough endoplasmic reticulum in 
      some cells, and WT31, recognizing alpha/beta framework, was mostly negative, 
      while CD3 was expressed in the majority of the cells. These facts indicate that 
      HTLV-I-infected cells were present in CSF of these two patients, and suggest that 
      neurological disorders associated with HTLV-I may not be restricted to myelopathy 
      and may include brain abnormalities.
FAU - Iwata, J
AU  - Iwata J
AD  - Department of Pathology, Kochi Medical School, Japan.
FAU - Oka, T
AU  - Oka T
FAU - Furihata, M
AU  - Furihata M
FAU - Sonobe, H
AU  - Sonobe H
FAU - Matsubayashi, K
AU  - Matsubayashi K
FAU - Uemura, Y
AU  - Uemura Y
FAU - Miyoshi, I
AU  - Miyoshi I
FAU - Ohtsuki, Y
AU  - Ohtsuki Y
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Aged
MH  - Antigens, Viral/analysis
MH  - Blotting, Southern
MH  - Brain Diseases/*microbiology
MH  - CD4-Positive T-Lymphocytes/*microbiology
MH  - Cell Line/*microbiology
MH  - HTLV-I Infections/*microbiology
MH  - Human T-lymphotropic virus 1/ultrastructure
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/*microbiology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1007/BF01311306 [doi]
PST - ppublish
SO  - Arch Virol. 1991;118(1-2):101-12. doi: 10.1007/BF01311306.