PMID- 16756374
OWN - NLM
STAT- MEDLINE
DCOM- 20060808
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 54
IP  - 12
DP  - 2006 Jun 14
TI  - Estrogenic potency of food-packaging-associated plasticizers and antioxidants as 
      detected in ERalpha and ERbeta reporter gene cell lines.
PG  - 4407-16
AB  - This study presents the estrogenic potency of 21 food-packaging-associated 
      compounds determined for the first time, using two transfected U2-OS (human 
      osteoblasts devoid of endogenous estrogen receptors) estrogen receptor (ER) alpha 
      and beta cell lines. Six plasticizers and three antioxidants were slightly 
      estrogenic in the ERalpha cells. The model compounds bisphenol A and nonylphenol, 
      one plasticizer [tris(2-ethylhexyl)trimellitate (TEHTM)], and two antioxidants 
      (propyl gallate and butylated hydroxyanisole) were estrogenic in both ERalpha and 
      ERbeta cells. Compared to estradiol (E2), these compounds appeared to be 
      relatively more estrogenic in the ERbeta cells than in the ERalpha cells. Three 
      sorbitol-based plasticizers activated neither ERalpha nor ERbeta and may be good 
      replacements of existing plasticizers. All responses were additive with the 
      response of E2. This indicates that they may contribute to the total effects of 
      the pool of estrogenic compounds humans are exposed to. The estrogenic potencies 
      of these compounds, together with the suggested beneficial effect of 
      ERbeta-mediated responses and adverse ERalpha-mediated effects, support the 
      importance of detecting characteristics for ERalpha and ERbeta response 
      separately in independent models, as done in the present study.
FAU - ter Veld, Marcel G R
AU  - ter Veld MG
AD  - Toxicology section, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The 
      Netherlands. marcel.terveld@wur.nl
FAU - Schouten, Bastiaan
AU  - Schouten B
FAU - Louisse, Jochem
AU  - Louisse J
FAU - van Es, Daan S
AU  - van Es DS
FAU - van der Saag, Paul T
AU  - van der Saag PT
FAU - Rietjens, Ivonne M C M
AU  - Rietjens IM
FAU - Murk, Albertinka J
AU  - Murk AJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antioxidants)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (Plasticizers)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Cell Line
MH  - Estradiol/pharmacology
MH  - Estrogen Receptor alpha/*drug effects/genetics
MH  - Estrogen Receptor beta/*drug effects/genetics
MH  - Estrogens/*pharmacology
MH  - *Food Packaging
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Osteoblasts
MH  - Plasticizers/*pharmacology
MH  - Transfection
EDAT- 2006/06/08 09:00
MHDA- 2006/08/09 09:00
CRDT- 2006/06/08 09:00
PHST- 2006/06/08 09:00 [pubmed]
PHST- 2006/08/09 09:00 [medline]
PHST- 2006/06/08 09:00 [entrez]
AID - 10.1021/jf052864f [doi]
PST - ppublish
SO  - J Agric Food Chem. 2006 Jun 14;54(12):4407-16. doi: 10.1021/jf052864f.